Phosphorylation of the Human Ubiquitin-conjugating Enzyme, CDC34, by Casein Kinase 2

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Abstract

The ubiquitin-conjugating enzyme, CDC34, has been implicated in the ubiquitination of a number of vertebrate substrates, including p27 Kip1, IKBα, Wee1, and MyoD. We show that mammalian CDC34 is a phospho-protein that is phosphorylated in proliferating cells. By yeast two-hybrid screening, we identified the regulatory (β) subunit of human casein kinase 2 (CK2) as a CDC34-interacting protein and show that human CDC34 inter-acts in vivo with CK2β in transfected cells, CDC34 is specifically phosphorylated in vitro by recombinant CK2 and HeLa nuclear extract at five sites within the carboxyl-terminal 36 amino acids of CDC34. Importantly, this phosphorylation is inhibited by heparin, a substrate-specific inhibitor of CK2. We have also identified a kinase activity associated with CDC34 in proliferating cells, and we show that this kinase is sensitive to heparin and can utilize GTP, strongly suggesting it is CK2. Phosphorylation of CDC34 by the associated kinase maps predominantly to residues 203 and 222. Mutation of CDC34 at CK2-targeted residues, Ser-203, Ser-222, Ser-231, Thr-233, and Ser-236, abolishes the phosphorylation of CDC34 observed in vivo and markedly shifts nuclearly localized CDC34 to the cytoplasm. These results suggest a potential role for CK2-mediated phosphorylation in the regulation of CDC34 cell localization and function.

Original languageEnglish (US)
Pages (from-to)41049-41058
Number of pages10
JournalJournal of Biological Chemistry
Volume276
Issue number44
DOIs
StatePublished - Nov 2 2001

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Ubiquitin-Conjugating Enzymes
Casein Kinase II
Phosphorylation
Ubiquitin
Phosphotransferases
Heparin
Ubiquitination
Substrates
Guanosine Triphosphate
Yeast
Vertebrates
Screening
Cytoplasm
Yeasts
Amino Acids
Mutation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Phosphorylation of the Human Ubiquitin-conjugating Enzyme, CDC34, by Casein Kinase 2. / Block, Karen; Boyer, Thomas G; Yew, Patricia R.

In: Journal of Biological Chemistry, Vol. 276, No. 44, 02.11.2001, p. 41049-41058.

Research output: Contribution to journalArticle

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