Abstract
Components of the extracellular matrix (ECM) of mammals have profound effects on the behavior and differentiation of many different cell types. Here, we report the results of biochemical and immunocytochemical investigations of the expression of SNAP-25 and phosphorylated neurofilament proteins (NFs) by cells grown on coverslips, cells cultured in EHS-ECM gels, and cells in situ in rat brain. SNAP-25 and phosphorylated NFs were detected by immunofluorescence in all these environments but were not detectable by Western analysis in extracts of cells grown on coverslips. The results support the interpretation that EHS-ECM induces differentiation of SH-SY5Y cells in culture and suggest this system as a model system for study of nerve tissue formation and repair.
Original language | English (US) |
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Pages (from-to) | 43-48 |
Number of pages | 6 |
Journal | Brain Research |
Volume | 934 |
Issue number | 1 |
DOIs | |
State | Published - Apr 26 2002 |
Externally published | Yes |
Keywords
- Developmental regulation
- Neuronal cytoskeleton
ASJC Scopus subject areas
- General Neuroscience
- Molecular Biology
- Clinical Neurology
- Developmental Biology