Phosphorylated neurofilaments and SNAP-25 in cultured SH-SY5Y neuroblastoma cells

Tracy L. Glass, Timothy D. Raabe, Dana M. García, Joseph R. Koke

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Components of the extracellular matrix (ECM) of mammals have profound effects on the behavior and differentiation of many different cell types. Here, we report the results of biochemical and immunocytochemical investigations of the expression of SNAP-25 and phosphorylated neurofilament proteins (NFs) by cells grown on coverslips, cells cultured in EHS-ECM gels, and cells in situ in rat brain. SNAP-25 and phosphorylated NFs were detected by immunofluorescence in all these environments but were not detectable by Western analysis in extracts of cells grown on coverslips. The results support the interpretation that EHS-ECM induces differentiation of SH-SY5Y cells in culture and suggest this system as a model system for study of nerve tissue formation and repair.

Original languageEnglish (US)
Pages (from-to)43-48
Number of pages6
JournalBrain Research
Volume934
Issue number1
DOIs
StatePublished - Apr 26 2002
Externally publishedYes

Keywords

  • Developmental regulation
  • Neuronal cytoskeleton

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint

Dive into the research topics of 'Phosphorylated neurofilaments and SNAP-25 in cultured SH-SY5Y neuroblastoma cells'. Together they form a unique fingerprint.

Cite this