TY - JOUR
T1 - Phosphoproteome analysis reveals new drought response and defense mechanisms of seedling leaves in bread wheat (Triticum aestivum L.)
AU - Zhang, Ming
AU - Lv, Dongwen
AU - Ge, Pei
AU - Bian, Yanwei
AU - Chen, Guanxing
AU - Zhu, Gengrui
AU - Li, Xiaohui
AU - Yan, Yueming
N1 - Funding Information:
This research was financially supported by the China–Australia Cooperation Project from Chinese Ministry of Science and Technology ( 2013DFG30530 ) and the National Key Project for Transgenic Crops of China ( 2011ZX08009-003-004 ).
PY - 2014/9/23
Y1 - 2014/9/23
N2 - Drought is a major form of abiotic stress that significantly affects plant growth and development. In this study, we performed the first phosphoproteome analysis of seedling leaves from two bread wheat cultivars (Hanxuan 10 and Ningchun 47) subjected to drought stress. As a result, a total of 191 and 251 unique phosphopeptides, representing 173 and 227 phosphoproteins in two cultivars, respectively, were identified as being significant changes in phosphorylation level (SCPL) under drought stress. Through the comparison of SCPL phosphoproteins between two cultivars, 31 common SCPL phosphoproteins were found in both cultivars. Function analysis showed that the SCPL phosphoproteins in the two cultivars are mainly involved in three biological processes: RNA transcription/processing, stress/detoxification/defense, and signal transduction. Further analyses revealed that some SCPL phosphoproteins may play key roles in signal transduction and the signaling cascade under drought stress. Furthermore, some phosphoproteins related to drought tolerance and osmotic regulation exhibited significant phosphorylation changes. This study used a series of bioinformatics tools to profile the phosphorylation status of wheat seedling leaves under drought stress with greater accuracy. Biological significance: Drought is of the most studied abiotic stresses, because it severely restricts the development and yield of plants. In this study, large numbers of stress-related phosphoproteins are identified from the two bread wheat cultivars. These phosphoproteins contribute to signal transduction, osmotic regulation and ROS scavenging under water stress. This work provides a detailed insight into the mechanisms of drought response and defense in bread wheat from the perspective of phosphoproteomics, and identifies some important drought-tolerant candidates for further transgenosis study and incorporation into the breeding of resistant cultivars.
AB - Drought is a major form of abiotic stress that significantly affects plant growth and development. In this study, we performed the first phosphoproteome analysis of seedling leaves from two bread wheat cultivars (Hanxuan 10 and Ningchun 47) subjected to drought stress. As a result, a total of 191 and 251 unique phosphopeptides, representing 173 and 227 phosphoproteins in two cultivars, respectively, were identified as being significant changes in phosphorylation level (SCPL) under drought stress. Through the comparison of SCPL phosphoproteins between two cultivars, 31 common SCPL phosphoproteins were found in both cultivars. Function analysis showed that the SCPL phosphoproteins in the two cultivars are mainly involved in three biological processes: RNA transcription/processing, stress/detoxification/defense, and signal transduction. Further analyses revealed that some SCPL phosphoproteins may play key roles in signal transduction and the signaling cascade under drought stress. Furthermore, some phosphoproteins related to drought tolerance and osmotic regulation exhibited significant phosphorylation changes. This study used a series of bioinformatics tools to profile the phosphorylation status of wheat seedling leaves under drought stress with greater accuracy. Biological significance: Drought is of the most studied abiotic stresses, because it severely restricts the development and yield of plants. In this study, large numbers of stress-related phosphoproteins are identified from the two bread wheat cultivars. These phosphoproteins contribute to signal transduction, osmotic regulation and ROS scavenging under water stress. This work provides a detailed insight into the mechanisms of drought response and defense in bread wheat from the perspective of phosphoproteomics, and identifies some important drought-tolerant candidates for further transgenosis study and incorporation into the breeding of resistant cultivars.
KW - Bread wheat
KW - Drought stress
KW - Label-free quantitative
KW - Phosphoproteome
KW - Response and defense
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U2 - 10.1016/j.jprot.2014.07.010
DO - 10.1016/j.jprot.2014.07.010
M3 - Article
C2 - 25065648
AN - SCOPUS:84905443201
SN - 1874-3919
VL - 109
SP - 290
EP - 308
JO - Journal of Proteomics
JF - Journal of Proteomics
ER -