Pharmacological studies on the regulation of N-acetyltransferase activity and melatonin content of the pineal gland of the Syrian hamster

S. Steinlechner, Thomas S King, T. H. Champney, B. A. Richardson, Russel J Reiter

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Thus far, all attempts to stimulate melatonin synthesis by β-adrenergic receptor agonists in the Syrian hamster pineal gland have failed. Neither a wide range of dosages of isoproterenol (0.5 mg/kg to 24 mg/kg), nor prolonged treatment with norepinephrine, the natural neurotransmitter, increased N-acetyltransferase (NAT) activity or melatonin production. In the present study, the administration of isoproterenol at night was likewise ineffective in advancing or enhancing the normal nightly melatonin peak. Also, we did not find a delayed effect 7 or 8 h after the administration of the drug. Furthermore, we tested the idea of coneurotransmitters such as octopamine or dopamine being possibly necessary for stimulation, but could not find any effect of these substances on melatonin synthesis. In addition, a parasympatholytic agent, atropine, did not increase the responsiveness to sympathomimetic agents. Administration of a phosphodiesterase inhibitor was also ineffective in stimulating NAT activity. On the other hand, isoproterenol did retard the drop in NAT and melatonin after lights-on at night, indicating that β-receptors are involved in maintaining elevated melatonin levels.

Original languageEnglish (US)
Pages (from-to)109-119
Number of pages11
JournalJournal of Pineal Research
Volume2
Issue number2
DOIs
StatePublished - 1985

Fingerprint

Acetyltransferases
Pineal Gland
Mesocricetus
Melatonin
Pharmacology
Isoproterenol
Octopamine
Parasympatholytics
Adrenergic Agonists
Sympathomimetics
Phosphodiesterase Inhibitors
Atropine
Neurotransmitter Agents
Dopamine
Norepinephrine
Light
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Endocrinology

Cite this

Pharmacological studies on the regulation of N-acetyltransferase activity and melatonin content of the pineal gland of the Syrian hamster. / Steinlechner, S.; King, Thomas S; Champney, T. H.; Richardson, B. A.; Reiter, Russel J.

In: Journal of Pineal Research, Vol. 2, No. 2, 1985, p. 109-119.

Research output: Contribution to journalArticle

@article{635d0c6b65e5434db11e10954168606c,
title = "Pharmacological studies on the regulation of N-acetyltransferase activity and melatonin content of the pineal gland of the Syrian hamster",
abstract = "Thus far, all attempts to stimulate melatonin synthesis by β-adrenergic receptor agonists in the Syrian hamster pineal gland have failed. Neither a wide range of dosages of isoproterenol (0.5 mg/kg to 24 mg/kg), nor prolonged treatment with norepinephrine, the natural neurotransmitter, increased N-acetyltransferase (NAT) activity or melatonin production. In the present study, the administration of isoproterenol at night was likewise ineffective in advancing or enhancing the normal nightly melatonin peak. Also, we did not find a delayed effect 7 or 8 h after the administration of the drug. Furthermore, we tested the idea of coneurotransmitters such as octopamine or dopamine being possibly necessary for stimulation, but could not find any effect of these substances on melatonin synthesis. In addition, a parasympatholytic agent, atropine, did not increase the responsiveness to sympathomimetic agents. Administration of a phosphodiesterase inhibitor was also ineffective in stimulating NAT activity. On the other hand, isoproterenol did retard the drop in NAT and melatonin after lights-on at night, indicating that β-receptors are involved in maintaining elevated melatonin levels.",
author = "S. Steinlechner and King, {Thomas S} and Champney, {T. H.} and Richardson, {B. A.} and Reiter, {Russel J}",
year = "1985",
doi = "10.1111/j.1600-079X.1985.tb00632.x",
language = "English (US)",
volume = "2",
pages = "109--119",
journal = "Journal of Pineal Research",
issn = "0742-3098",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Pharmacological studies on the regulation of N-acetyltransferase activity and melatonin content of the pineal gland of the Syrian hamster

AU - Steinlechner, S.

AU - King, Thomas S

AU - Champney, T. H.

AU - Richardson, B. A.

AU - Reiter, Russel J

PY - 1985

Y1 - 1985

N2 - Thus far, all attempts to stimulate melatonin synthesis by β-adrenergic receptor agonists in the Syrian hamster pineal gland have failed. Neither a wide range of dosages of isoproterenol (0.5 mg/kg to 24 mg/kg), nor prolonged treatment with norepinephrine, the natural neurotransmitter, increased N-acetyltransferase (NAT) activity or melatonin production. In the present study, the administration of isoproterenol at night was likewise ineffective in advancing or enhancing the normal nightly melatonin peak. Also, we did not find a delayed effect 7 or 8 h after the administration of the drug. Furthermore, we tested the idea of coneurotransmitters such as octopamine or dopamine being possibly necessary for stimulation, but could not find any effect of these substances on melatonin synthesis. In addition, a parasympatholytic agent, atropine, did not increase the responsiveness to sympathomimetic agents. Administration of a phosphodiesterase inhibitor was also ineffective in stimulating NAT activity. On the other hand, isoproterenol did retard the drop in NAT and melatonin after lights-on at night, indicating that β-receptors are involved in maintaining elevated melatonin levels.

AB - Thus far, all attempts to stimulate melatonin synthesis by β-adrenergic receptor agonists in the Syrian hamster pineal gland have failed. Neither a wide range of dosages of isoproterenol (0.5 mg/kg to 24 mg/kg), nor prolonged treatment with norepinephrine, the natural neurotransmitter, increased N-acetyltransferase (NAT) activity or melatonin production. In the present study, the administration of isoproterenol at night was likewise ineffective in advancing or enhancing the normal nightly melatonin peak. Also, we did not find a delayed effect 7 or 8 h after the administration of the drug. Furthermore, we tested the idea of coneurotransmitters such as octopamine or dopamine being possibly necessary for stimulation, but could not find any effect of these substances on melatonin synthesis. In addition, a parasympatholytic agent, atropine, did not increase the responsiveness to sympathomimetic agents. Administration of a phosphodiesterase inhibitor was also ineffective in stimulating NAT activity. On the other hand, isoproterenol did retard the drop in NAT and melatonin after lights-on at night, indicating that β-receptors are involved in maintaining elevated melatonin levels.

UR - http://www.scopus.com/inward/record.url?scp=0021799673&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021799673&partnerID=8YFLogxK

U2 - 10.1111/j.1600-079X.1985.tb00632.x

DO - 10.1111/j.1600-079X.1985.tb00632.x

M3 - Article

VL - 2

SP - 109

EP - 119

JO - Journal of Pineal Research

JF - Journal of Pineal Research

SN - 0742-3098

IS - 2

ER -