A methanolic extract of W. somnifera root inhibited the specific binding of [3H]GABA and [35S]TBPS, and enhanced the binding of [3H]flunitrazepam to their putative receptor sites. The extract (5 μg) inhibited [3H]GABA binding by 20 ± 6 per cent whereas a concentration of 1 mg of the extract produced 100 per cent inhibition. The extract (5-100 μg) produced 20 ± 4 to 91 ± 16 per cent enhancement of [3H]flunitrazepam binding. In functional studies using 36Cl-influx assay in mammalian spinal cord neurons, w. somnifera root extract increased 36Cl-influx in the absence of GABA. This effect on 36Cl-influx was blocked by bicuculline and picrotoxin; and enhanced by diazepam. These results suggest that the W. somnifera extract contains an ingredient which has a GABA-mietic activity.
|Original language||English (US)|
|Number of pages||4|
|Journal||Indian Journal of Medical Research - Section B Biomedical Research Other Than Infectious Diseases|
|State||Published - 1991|
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