Pharmacological effects of Withania somnifera root extract on GABA(A) receptor complex

A. K. Mehta, P. Binkley, S. S. Gandhi, M. K. Ticku

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82 Scopus citations

Abstract

A methanolic extract of W. somnifera root inhibited the specific binding of [3H]GABA and [35S]TBPS, and enhanced the binding of [3H]flunitrazepam to their putative receptor sites. The extract (5 μg) inhibited [3H]GABA binding by 20 ± 6 per cent whereas a concentration of 1 mg of the extract produced 100 per cent inhibition. The extract (5-100 μg) produced 20 ± 4 to 91 ± 16 per cent enhancement of [3H]flunitrazepam binding. In functional studies using 36Cl-influx assay in mammalian spinal cord neurons, w. somnifera root extract increased 36Cl-influx in the absence of GABA. This effect on 36Cl-influx was blocked by bicuculline and picrotoxin; and enhanced by diazepam. These results suggest that the W. somnifera extract contains an ingredient which has a GABA-mietic activity.

Original languageEnglish (US)
Pages (from-to)312-315
Number of pages4
JournalIndian Journal of Medical Research - Section B Biomedical Research Other Than Infectious Diseases
Volume94
Issue numberAUG.
StatePublished - 1991

ASJC Scopus subject areas

  • Biochemistry

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