PGC-1α-induced mitochondrial alterations in 3T3 fibroblast cells

Huiyun Liang, Yidong Bai, Youfen Li, Arlan Richardson, Walter F. Ward

Research output: Chapter in Book/Report/Conference proceedingConference contribution

12 Scopus citations

Abstract

Peroxisome proliferation activator receptor (PPAR) γ-coactivator 1α (PGC-1α), a transcription coactivator, functions as a master regulator of a wide array of metabolic and physiological processes and is an essential factor in the process of mitochondrial biogenesis. Transfection of NIH 3T3 fibroblasts with a mouse cDNA for PGC-1α led to the induction of markers of mitochondrial biogenesis, that is, mitochondrial transcription factor A (mtTFA), cytochrome c, and mitochondrial DNA (mtDNA). Mitochondrial biogenesis-associated net protein synthesis appears to be accomplished by a reduction in the rate of mitochondrial protein degradation with little or no change in the rate of protein synthesis. Overexpression of PGC-1α did not adversely affect cellular proliferation. Cellular ATP levels were increased in the transfected cells and they were more resistant to oxidative stress than the control nontransfected 3T3 cells. This resistance to oxidative stress was manifested by both an improved viability and the maintenance of mitochondrial membrane potential in the transfected cells when exposed to t-butyl hydroperoxide (t-BOOH). It therefore appears that PGC-1α overexpression stimulates mitochondrial biogenesis in 3T3 cells making them more resistant to oxidative stressors.

Original languageEnglish (US)
Title of host publicationBiogerontology
Subtitle of host publicationMechanisms and Interventions
PublisherBlackwell Publishing Inc.
Pages264-279
Number of pages16
ISBN (Print)1573316792, 9781573316798
DOIs
StatePublished - Apr 2007

Publication series

NameAnnals of the New York Academy of Sciences
Volume1100
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Mitochondrial biogenesis
  • Mitochondrial protein turnover
  • Oxidative stress
  • PGC-1α

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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