PGC-1α-induced mitochondrial alterations in 3T3 fibroblast cells

Huiyun Liang, Yidong Bai, Youfen Li, Arlan Richardson, Walter F. Ward

Research output: Chapter in Book/Report/Conference proceedingConference contribution

12 Citations (Scopus)

Abstract

Peroxisome proliferation activator receptor (PPAR) γ-coactivator 1α (PGC-1α), a transcription coactivator, functions as a master regulator of a wide array of metabolic and physiological processes and is an essential factor in the process of mitochondrial biogenesis. Transfection of NIH 3T3 fibroblasts with a mouse cDNA for PGC-1α led to the induction of markers of mitochondrial biogenesis, that is, mitochondrial transcription factor A (mtTFA), cytochrome c, and mitochondrial DNA (mtDNA). Mitochondrial biogenesis-associated net protein synthesis appears to be accomplished by a reduction in the rate of mitochondrial protein degradation with little or no change in the rate of protein synthesis. Overexpression of PGC-1α did not adversely affect cellular proliferation. Cellular ATP levels were increased in the transfected cells and they were more resistant to oxidative stress than the control nontransfected 3T3 cells. This resistance to oxidative stress was manifested by both an improved viability and the maintenance of mitochondrial membrane potential in the transfected cells when exposed to t-butyl hydroperoxide (t-BOOH). It therefore appears that PGC-1α overexpression stimulates mitochondrial biogenesis in 3T3 cells making them more resistant to oxidative stressors.

Original languageEnglish (US)
Title of host publicationAnnals of the New York Academy of Sciences
Pages264-279
Number of pages16
Volume1100
DOIs
StatePublished - Apr 2007

Publication series

NameAnnals of the New York Academy of Sciences
Volume1100
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

3T3 Cells
Oxidative stress
Organelle Biogenesis
Fibroblasts
Cells
tert-Butylhydroperoxide
Mitochondrial Proteins
Transcription
Cytochromes c
Mitochondrial DNA
Proteins
Oxidative Stress
Complementary DNA
Adenosine Triphosphate
Physiological Phenomena
Membranes
Degradation
Peroxisomes
Mitochondrial Membrane Potential
Proteolysis

Keywords

  • Mitochondrial biogenesis
  • Mitochondrial protein turnover
  • Oxidative stress
  • PGC-1α

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Liang, H., Bai, Y., Li, Y., Richardson, A., & Ward, W. F. (2007). PGC-1α-induced mitochondrial alterations in 3T3 fibroblast cells. In Annals of the New York Academy of Sciences (Vol. 1100, pp. 264-279). (Annals of the New York Academy of Sciences; Vol. 1100). https://doi.org/10.1196/annals.1395.028

PGC-1α-induced mitochondrial alterations in 3T3 fibroblast cells. / Liang, Huiyun; Bai, Yidong; Li, Youfen; Richardson, Arlan; Ward, Walter F.

Annals of the New York Academy of Sciences. Vol. 1100 2007. p. 264-279 (Annals of the New York Academy of Sciences; Vol. 1100).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Liang, H, Bai, Y, Li, Y, Richardson, A & Ward, WF 2007, PGC-1α-induced mitochondrial alterations in 3T3 fibroblast cells. in Annals of the New York Academy of Sciences. vol. 1100, Annals of the New York Academy of Sciences, vol. 1100, pp. 264-279. https://doi.org/10.1196/annals.1395.028
Liang H, Bai Y, Li Y, Richardson A, Ward WF. PGC-1α-induced mitochondrial alterations in 3T3 fibroblast cells. In Annals of the New York Academy of Sciences. Vol. 1100. 2007. p. 264-279. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1395.028
Liang, Huiyun ; Bai, Yidong ; Li, Youfen ; Richardson, Arlan ; Ward, Walter F. / PGC-1α-induced mitochondrial alterations in 3T3 fibroblast cells. Annals of the New York Academy of Sciences. Vol. 1100 2007. pp. 264-279 (Annals of the New York Academy of Sciences).
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