TY - JOUR
T1 - Peroxidase H2O2 halide system
T2 - cytotoxic effect on mammalian tumor cells
AU - Clark, R. A.
AU - Klebanoff, S. J.
AU - Einstein, A. B.
AU - Fefer, A.
PY - 1975
Y1 - 1975
N2 - Myeloperoxidase, H2O2, and a halide constitute a potent antimicrobial system. A cytotoxic effect of this system on a line of mouse ascitic lymphoma cells (LSTRA) is demonstrated here using 4 different assay systems: 51Cr release, trypan blue exclusion, inhibition of glucose C 1 oxidation, and loss of oncogenicity for mice. Deletion of each component of the system, preheating the peroxidase, or addition of azide, cyanide, or catalase abolished the cytotoxicity. Myeloperoxidase was effective with either chloride or iodide as the halide, while lactoperoxidase was effective with iodide but not chloride. The iodinated thyroid hormones, triiodothyronine and thyroxine, could substitute for the the halide, and H2O2 could be replaced by a peroxide generating enzyme system such as glucose and glucose oxidase or by H2O2 producing bacteria such as pneumococci or streptococci. The possibility is raised that the peroxidases of inflammatory cells and certain biologic fluids may affect tumor initiation or growth in vivo.
AB - Myeloperoxidase, H2O2, and a halide constitute a potent antimicrobial system. A cytotoxic effect of this system on a line of mouse ascitic lymphoma cells (LSTRA) is demonstrated here using 4 different assay systems: 51Cr release, trypan blue exclusion, inhibition of glucose C 1 oxidation, and loss of oncogenicity for mice. Deletion of each component of the system, preheating the peroxidase, or addition of azide, cyanide, or catalase abolished the cytotoxicity. Myeloperoxidase was effective with either chloride or iodide as the halide, while lactoperoxidase was effective with iodide but not chloride. The iodinated thyroid hormones, triiodothyronine and thyroxine, could substitute for the the halide, and H2O2 could be replaced by a peroxide generating enzyme system such as glucose and glucose oxidase or by H2O2 producing bacteria such as pneumococci or streptococci. The possibility is raised that the peroxidases of inflammatory cells and certain biologic fluids may affect tumor initiation or growth in vivo.
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U2 - 10.1182/blood.v45.2.161.bloodjournal452161
DO - 10.1182/blood.v45.2.161.bloodjournal452161
M3 - Article
C2 - 1120184
AN - SCOPUS:0016593626
SN - 0006-4971
VL - 45
SP - 161
EP - 170
JO - Blood
JF - Blood
IS - 2
ER -