Perivascular Fibroblasts of the Developing Spleen Act as LTα1β2-Dependent Precursors of Both T and B Zone Organizer Cells

Karin Schaeuble, Mirjam R. Britschgi, Leo Scarpellino, Stéphanie Favre, Ying Xu, Ekaterina Koroleva, Tonje K.A. Lissandrin, Alexander Link, Mehrdad Matloubian, Carl F. Ware, Sergei A. Nedospasov, Alexei V. Tumanov, Jason G. Cyster, Sanjiv A. Luther

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

T and B cell compartmentalization is a hallmark of secondary lymphoid organs and is maintained by chemokine-expressing stromal cells. How this stromal cell network initially develops and differentiates into two distinct subsets is poorly known, especially for the splenic white pulp (WP). Here, we show that perivascular fibroblast precursors are triggered by LTα1β2 signals to expand, express CCL19/21, and then differentiate into two functionally distinct fibroblast subsets responsible for B and T cell clustering and WP compartmentalization. Failure to express or sense CCL19 leads to impaired T zone development, while lack of B cells or LTα1β2 leads to an earlier and stronger impairment in WP development. We therefore propose that WP development proceeds in multiple steps, with LTα1β2+ B cells acting as major inducer cells driving the expansion and gradual differentiation of perivascular fibroblasts into T and B zone organizer cells. Schaeuble et al. describe the postnatal development and compartmentalization of splenic white pulp cords in mice, with an emphasis on stromal organizer cells. These cells are identified as fibroblasts that, upon interaction with LTα1β2+ B cells, differentiate into two subsets that cluster T and B cells into separate compartments.

Original languageEnglish (US)
Pages (from-to)2500-2514
Number of pages15
JournalCell Reports
Volume21
Issue number9
DOIs
StatePublished - Nov 28 2017

Keywords

  • CCR7
  • CXCL13
  • ILC3
  • LTi cells
  • PALS
  • chemokines
  • fibroblast heterogeneity
  • lymphocytes
  • lymphotoxin
  • stroma

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Schaeuble, K., Britschgi, M. R., Scarpellino, L., Favre, S., Xu, Y., Koroleva, E., Lissandrin, T. K. A., Link, A., Matloubian, M., Ware, C. F., Nedospasov, S. A., Tumanov, A. V., Cyster, J. G., & Luther, S. A. (2017). Perivascular Fibroblasts of the Developing Spleen Act as LTα1β2-Dependent Precursors of Both T and B Zone Organizer Cells. Cell Reports, 21(9), 2500-2514. https://doi.org/10.1016/j.celrep.2017.10.119