Peripheral nervous system-specific genes identified by subtractive cDNA cloning

Armen N Akopian, J. N. Wood

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

An improved method for constructing and screening subtractive cDNA libraries has been used to identify 46 mRNA transcripts that are expressed selectively in neonatal rat dorsal root ganglia (DRG) as judged by Northern blots and in situ hybridization. Sequence analysis demonstrates that both known (e.g. peripherin, calcitonin gene-related peptide, myelin P0) and novel identifiable transcripts (e.g. C-protein-like, synuclein-like, villin-like) are present in the library. Half of the transcripts (23) are undetectable in liver, kidney, heart, spleen, cerebellum, and cerebral cortex. Of the DRG- specific transcripts, 12 contain putative open reading frames that show no identity with known proteins. The construction of such a subtractive library thus provides us with both known and novel markers, and identifies new predicted DRG-specific proteins. In addition, the DRG-specific clones provide probes to define the regulatory elements that specify peripheral nervous- system-specific gene expression.

Original languageEnglish (US)
Pages (from-to)21264-21270
Number of pages7
JournalJournal of Biological Chemistry
Volume270
Issue number36
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Cloning
Peripheral Nervous System
Spinal Ganglia
Neurology
Organism Cloning
Complementary DNA
Genes
Synucleins
Peripherins
Calcitonin Gene-Related Peptide
Protein C
Gene Library
Gene expression
Liver
Libraries
Rats
Screening
Proteins
Messenger RNA
Myelin Sheath

ASJC Scopus subject areas

  • Biochemistry

Cite this

Peripheral nervous system-specific genes identified by subtractive cDNA cloning. / Akopian, Armen N; Wood, J. N.

In: Journal of Biological Chemistry, Vol. 270, No. 36, 1995, p. 21264-21270.

Research output: Contribution to journalArticle

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