TY - JOUR
T1 - Perfusion preservation of rodent kidneys in a portable preservation device based on fluidics technology
AU - Tolstykh, Gleb P.
AU - Gelineau, Jerry F.
AU - Bunegin, Leonid
PY - 2002/5/15
Y1 - 2002/5/15
N2 - Background. Technology that can implement the basic requirements for successful organ preservation in a portable configuration has yet to be realized. Methods. This work evaluates kidney preservation in a new class of portable organ preservation technology based on fluidics principles. During hypothermic pulsatile perfusion preservation (HPPP), oxygen consumption, renal vascular resistance (RVR), pH, pCO2, and perfusion pressure were measured. After 24 hr of preservation, perfusate distribution was assessed, and oxygen consumption, RVR, and glomerular filtration rate (GFR) were compared in perfused, statically stored, and freshly harvested kidneys. Results. During HPPP, perfusion pressure was 5.8±3.3 mmHg with oxygen delivery to the organs in excess of 3.5 times the organ metabolic requirement. During function measurements, RVR was not statistically different in the three groups; however, both oxygen consumption and GFR in the statically stored organs were significantly lower than in HPPP stored or freshly harvested kidneys. Conclusions. Our findings suggest that full portability in a hypothermic perfusion preservation device seems feasible utilizing fluidics-based technology.
AB - Background. Technology that can implement the basic requirements for successful organ preservation in a portable configuration has yet to be realized. Methods. This work evaluates kidney preservation in a new class of portable organ preservation technology based on fluidics principles. During hypothermic pulsatile perfusion preservation (HPPP), oxygen consumption, renal vascular resistance (RVR), pH, pCO2, and perfusion pressure were measured. After 24 hr of preservation, perfusate distribution was assessed, and oxygen consumption, RVR, and glomerular filtration rate (GFR) were compared in perfused, statically stored, and freshly harvested kidneys. Results. During HPPP, perfusion pressure was 5.8±3.3 mmHg with oxygen delivery to the organs in excess of 3.5 times the organ metabolic requirement. During function measurements, RVR was not statistically different in the three groups; however, both oxygen consumption and GFR in the statically stored organs were significantly lower than in HPPP stored or freshly harvested kidneys. Conclusions. Our findings suggest that full portability in a hypothermic perfusion preservation device seems feasible utilizing fluidics-based technology.
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U2 - 10.1097/00007890-200205150-00023
DO - 10.1097/00007890-200205150-00023
M3 - Article
C2 - 12023632
AN - SCOPUS:0037093426
VL - 73
SP - 1508
EP - 1510
JO - Transplantation
JF - Transplantation
SN - 0041-1337
IS - 9
ER -