Perfusion and diffusion imaging in acute focal cerebral ischemia: Temporal vs. spatial resolution

Juergen Bardutzky, Qiang Shen, James Bouley, Christopher H. Sotak, Timothy Q Duong, Marc Fisher

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


High-resolution diffusion- (DWI) and perfusion-weighted (PWI) imaging may provide substantial benefits in accurate delineation of normal, ischemic, and at-risk tissue. We compared the capability of low (400 × 400 μm 2) and high (200 × 200 μm2) spatial resolution imaging in characterizing the spatiotemporal evolution of the ischemic lesion in a permanent middle artery occlusion (MCAO) model in rats. Serial measurements of cerebral blood flow (CBF) and the apparent diffusion coefficient (ADC) were performed. Lesion volumes were calculated by using viability thresholds or by visual inspection, and correlated with infarct volume defined by TTC staining at 24 h after MCAO. At the very early phase of ischemia, high-resolution resulted in a significantly larger ADC-derived lesion volume and a smaller PWI/DWI mismatch. At 3 h after MCAO, ADC and CBF lesions showed similar robust correlations with TTC-defined infarct volumes for both groups using previously established thresholds. When lesions were determined visually, low-resolution resulted in a substantial overestimation of TTC-defined infarct volume and a lower inter-observer reliability (r = 0.75), whereas high-resolution produced an excellent correlation with TTC-defined infarct volume and inter-observer reliability (r = 0.96). In conclusion, high-resolution MRI resulted in substantial temporal averaging of the ischemic lesion during the early phase, but was clearly superior in visual determination of final infarct size. Low-resolution reasonably evaluated the temporal and spatial evolution of ischemia when thresholds were used.

Original languageEnglish (US)
Pages (from-to)155-162
Number of pages8
JournalBrain Research
Issue number1-2
StatePublished - May 10 2005
Externally publishedYes


  • ADC
  • CBF
  • Focal ischemia
  • High-resolution imaging
  • Perfusion-diffusion mismatch

ASJC Scopus subject areas

  • Clinical Neurology
  • Molecular Biology
  • General Neuroscience
  • Developmental Biology


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