Peptides identified through phage display direct immunogenic antigen to dendritic cells

Tyler J. Curiel, Cindy Morris, Michael Brumlik, Samuel J. Landry, Kristiaan Finstad, Anne Nelson, Virendra Joshi, Christopher Hawkins, Xavier Alarez, Andrew Lackner, Mansour Mohamadzadeh

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

Dendritic cells (DC) play a critical role in adaptive immunity by presenting Ag, thereby priming naive T cells. Specific DC-binding peptides were identified using a phage display peptide library. DC-peptides were fused to hepatitis C virus nonstructural protein 3 (NS3) while preserving DC targeting selectivity and Ag immunogenity. The NS3-DC-peptide fusion protein was efficiently presented to CD4+ and CD8+ T cells derived from hepatitis C virus-positive blood cells, inducing their activation and proliferation. This immunogenic fusion protein was significantly more potent than NS3 control fusion protein or NS3 alone. In chimeric NOD-SCID mice transplanted with human cells, DC-targeted NS3 primed naive CD4+ and CD8 T cells for potent NS3-specific proliferation and cytokine secretion. The capacity of peptides to specifically target immunogenic Ags to DC may establish a novel strategy for vaccine development.

Original languageEnglish (US)
Pages (from-to)7425-7431
Number of pages7
JournalJournal of Immunology
Volume172
Issue number12
DOIs
StatePublished - Jun 15 2004
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Curiel, T. J., Morris, C., Brumlik, M., Landry, S. J., Finstad, K., Nelson, A., Joshi, V., Hawkins, C., Alarez, X., Lackner, A., & Mohamadzadeh, M. (2004). Peptides identified through phage display direct immunogenic antigen to dendritic cells. Journal of Immunology, 172(12), 7425-7431. https://doi.org/10.4049/jimmunol.172.12.7425