Peptide methionine sulfoxide reductase (MsrA) is a virulence determinant in Mycoplasma genitalium

S. Dhandayuthapani, M. W. Blaylock, C. M. Bebear, W. G. Rasmussen, J. B. Baseman

Research output: Contribution to journalArticle

84 Scopus citations

Abstract

Mycoplasma genitalium is the smallest self-replicating microorganism and is implicated in human diseases, including urogenital and respiratory infections and arthritides. M. genitalium colonizes host cells primarily through adherence mechanisms mediated by a network of surface-associated membrane proteins, including adhesins and cytadherence-related proteins. In this paper, we show that cytadherence in M. genitalium is affected by an unrelated protein known as peptide methionine sulfoxide reductase (MsrA), an antioxidant repair enzyme that catalyzes the reduction of methionine sulfoxide [Met(O)] residues in proteins to methionine. An msrA disruption mutant of M. genitalium, constructed through homologous recombination, displayed markedly reduced adherence to sheep erythrocytes. In addition, the msrA mutant was incapable of growing in hamsters and exhibited hypersensitivity to hydrogen peroxide when compared to wild-type virulent M. genitalium. These results indicate that MsrA plays an important role in M. genitalium pathogenicity, possibly by protecting mycoplasma protein structures from oxidative damage or through alternate virulence-related pathways.

Original languageEnglish (US)
Pages (from-to)5645-5650
Number of pages6
JournalJournal of bacteriology
Volume183
Issue number19
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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