Peptide-coated vascular grafts: An in vivo study in sheep

Cheng Li, Arthur Hill, Mir Imran, Fermin Tio

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The data on function and patency of prosthetic vascular grafts in various clinical settings are limited. The purpose of this in vivo study was to compare the function and patency of P15-coated expanded polytetrafluoroethylene (ePTFE) vascular grafts to uncoated ePTFE grafts in sheep. The P15 cell-binding peptide was covalently immobilized onto the surface of ePTFE grafts by a novel atmospheric plasma coating method. We evaluated the amount of neointimal tissue ingrowth present at the arterial and venous sides of the anastomoses and the degree of endothelial cell resurfacing of the luminal surface of the graft. Four P15-coated grafts and two control grafts were implanted as arteriovenous grafts between the femoral artery and vein and the carotid artery and jugular vein in two sheep (n = 6). One animal was euthanized after 14 days and the other after 28 days. The study showed the intimal ingrowth was significantly less. The average intimal thickness of P15-coated grafts (658 μm) was approximately two and a half times less than that of uncoated samples (1657 μm). The newly formed endothelial cell lining was thicker and its coverage was more uniform for P15-coated grafts compared to the uncoated controls.

Original languageEnglish (US)
Pages (from-to)360-367
Number of pages8
JournalHemodialysis International
Volume8
Issue number4
DOIs
StatePublished - Oct 2004

Keywords

  • Arteriovenous grafts
  • Cell-binding peptides
  • ePTFE
  • Intimal tissue ingrowth
  • Vascular grafts

ASJC Scopus subject areas

  • Hematology
  • Nephrology

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