TY - JOUR
T1 - Pentose phosphate pathway in the scyphistomae of Chrysaora quinquecirrha
AU - Lin, Alan L.
AU - Zubkoff, Paul L.
PY - 1976
Y1 - 1976
N2 - 1. 1. Data obtained by two independent methods of analysis provide evidence for a functional pentose-phosphate pathway in the polyps of Chrysaora quinquecirrha. 2. 2. The first two enzymes of the pentose-phosphate pathway, glucose-6-phosphate dehydrogenase (E.C. 1.1.1.49) and 6-phosphogluconate dehydrogenase (E.C. 1.1.1.43), have single bands on polyacrylamide gel electrophoresis with relative mobilities of 0·20 and 0·47, respectively. 3. 3. Glucose-6-phosphate dehydrogenase is a particularly labile enzyme which must be stabilized by the addition of NADP during the extraction and electrophoresis procedures. 4. 4. The molar ratio of 14CO2 produced by the in vivo oxidation of [1-14C]-glucose and [6-14C]-glucose is greater than one, an observation consistent with a functional pentose-phosphate pathway.
AB - 1. 1. Data obtained by two independent methods of analysis provide evidence for a functional pentose-phosphate pathway in the polyps of Chrysaora quinquecirrha. 2. 2. The first two enzymes of the pentose-phosphate pathway, glucose-6-phosphate dehydrogenase (E.C. 1.1.1.49) and 6-phosphogluconate dehydrogenase (E.C. 1.1.1.43), have single bands on polyacrylamide gel electrophoresis with relative mobilities of 0·20 and 0·47, respectively. 3. 3. Glucose-6-phosphate dehydrogenase is a particularly labile enzyme which must be stabilized by the addition of NADP during the extraction and electrophoresis procedures. 4. 4. The molar ratio of 14CO2 produced by the in vivo oxidation of [1-14C]-glucose and [6-14C]-glucose is greater than one, an observation consistent with a functional pentose-phosphate pathway.
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U2 - 10.1016/0305-0491(76)90052-3
DO - 10.1016/0305-0491(76)90052-3
M3 - Article
C2 - 5232
AN - SCOPUS:0017282529
VL - 54
SP - 33
EP - 36
JO - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
JF - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
SN - 0305-0491
IS - 1
ER -