TY - JOUR
T1 - Pentose phosphate pathway in the scyphistomae of Chrysaora quinquecirrha
AU - Lin, Alan L.
AU - Zubkoff, Paul L.
N1 - Funding Information:
the polyps This indicates that most of the glucose was immediately metabolized after uptake instead of being stored m the organism's t~ssues The speculation that a closer evolutionary relationship between hydrozoans and scyphozoans than between scyphozoans and anthozoans due to the presence or absence of 6PGDH ~s not further advanced by these experLments The absence of 6PGDH in the hydrozoans (Rutherford & Lenhoff, 1969. Powers et al. 1968) and the total absence or presence of a hm-lted amount of 6PGDH present in the scyphozoans (Powers et al, 1968, this study), and the labile nature of 6PGDH, make it difficult to ascertain whether there ts a fairly large amount of 6PGDH in the hvmg cells The mdlcatlon of a strong PP-pathway present m the polyps of Chrysaora supported by the radiotracer data indicates that a reevaluation of the pentose-phosphate pathway in other coelenterates may be necessary Acknowledgements--We thank Dr R E Black and Mr J E Warlnner, III for stimulating discussions concerning the radlotracer techniques employed and for reviewing the manuscript, and Mrs P Crewe for maintaining the cultures This study was conducted as part of the Virginia Jellyfish Research Program under Public Law 89-720. The Jellyfish Act. Contract No N-043-226-72(G) from the Na-
PY - 1976
Y1 - 1976
N2 - 1. 1. Data obtained by two independent methods of analysis provide evidence for a functional pentose-phosphate pathway in the polyps of Chrysaora quinquecirrha. 2. 2. The first two enzymes of the pentose-phosphate pathway, glucose-6-phosphate dehydrogenase (E.C. 1.1.1.49) and 6-phosphogluconate dehydrogenase (E.C. 1.1.1.43), have single bands on polyacrylamide gel electrophoresis with relative mobilities of 0·20 and 0·47, respectively. 3. 3. Glucose-6-phosphate dehydrogenase is a particularly labile enzyme which must be stabilized by the addition of NADP during the extraction and electrophoresis procedures. 4. 4. The molar ratio of 14CO2 produced by the in vivo oxidation of [1-14C]-glucose and [6-14C]-glucose is greater than one, an observation consistent with a functional pentose-phosphate pathway.
AB - 1. 1. Data obtained by two independent methods of analysis provide evidence for a functional pentose-phosphate pathway in the polyps of Chrysaora quinquecirrha. 2. 2. The first two enzymes of the pentose-phosphate pathway, glucose-6-phosphate dehydrogenase (E.C. 1.1.1.49) and 6-phosphogluconate dehydrogenase (E.C. 1.1.1.43), have single bands on polyacrylamide gel electrophoresis with relative mobilities of 0·20 and 0·47, respectively. 3. 3. Glucose-6-phosphate dehydrogenase is a particularly labile enzyme which must be stabilized by the addition of NADP during the extraction and electrophoresis procedures. 4. 4. The molar ratio of 14CO2 produced by the in vivo oxidation of [1-14C]-glucose and [6-14C]-glucose is greater than one, an observation consistent with a functional pentose-phosphate pathway.
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U2 - 10.1016/0305-0491(76)90052-3
DO - 10.1016/0305-0491(76)90052-3
M3 - Article
C2 - 5232
AN - SCOPUS:0017282529
VL - 54
SP - 33
EP - 36
JO - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
JF - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
SN - 0305-0491
IS - 1
ER -