PDGF up-regulates CSF-1 gene transcription in ameloblast-like cells

Y. Wittrant, B. Sriniketan Bhandari, H. Abboud, N. Benson, K. Woodruff, M. MacDougall, S. Abboud-Werner

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Macrophage colony-stimulating factor (CSF-I) is a key regulatory cytokine for amelogenesis, and ameloblasts synthesize CSF-I. We hypothesized that PDGF stimulates DNA synthesis and regulates CSF-I in these cells. We determined the effect of PDGF on CSF-I expression using MEOE-3M ameloblasts as a model. By RT-PCR, MEOE-3M expressed PDGFRs and PDGF A- and B-chain mRNAs. PDGF-BB increased DNA synthesis and up-regulated CSF-I mRNA and protein in MEOE-3M. Cells transfected with CSF1 promoter deletion constructs were analyzed. A PDGF-responsive region between - 1.7 and - 0.795 kb, containing a consensus Pea3 binding motif, was identified. Electrophoretic mobility shift assay (EMSA) showed that PDGF-BB stimulated protein binding to this motif that was inhibited in the presence of anti-Pea3 antibody. Analysis of these data provides the first evidence that PDGFBB is a mitogen for MEOE-3M and increases CSF-I protein levels, predominantly by transcription. Elucidation of the cellular pathways that control CSF-1 expression may provide novel strategies for the regulation of enamel matrix formation.

Original languageEnglish (US)
Pages (from-to)33-38
Number of pages6
JournalJournal of Dental Research
Volume87
Issue number1
DOIs
StatePublished - Jan 2008

Fingerprint

Ameloblasts
Macrophage Colony-Stimulating Factor
Amelogenesis
Up-Regulation
Messenger RNA
DNA
Electrophoretic Mobility Shift Assay
Dental Enamel
Mitogens
Protein Binding
Genes
Anti-Idiotypic Antibodies
Proteins
Cytokines
Polymerase Chain Reaction
platelet-derived growth factor BB
platelet-derived growth factor A

Keywords

  • Ameloblasts
  • Gene transcription
  • Macrophage colony-stimulating factor
  • Platelet-derived growth factor
  • Transcription factors

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Wittrant, Y., Bhandari, B. S., Abboud, H., Benson, N., Woodruff, K., MacDougall, M., & Abboud-Werner, S. (2008). PDGF up-regulates CSF-1 gene transcription in ameloblast-like cells. Journal of Dental Research, 87(1), 33-38. https://doi.org/10.1177/154405910808700105

PDGF up-regulates CSF-1 gene transcription in ameloblast-like cells. / Wittrant, Y.; Bhandari, B. Sriniketan; Abboud, H.; Benson, N.; Woodruff, K.; MacDougall, M.; Abboud-Werner, S.

In: Journal of Dental Research, Vol. 87, No. 1, 01.2008, p. 33-38.

Research output: Contribution to journalArticle

Wittrant, Y, Bhandari, BS, Abboud, H, Benson, N, Woodruff, K, MacDougall, M & Abboud-Werner, S 2008, 'PDGF up-regulates CSF-1 gene transcription in ameloblast-like cells', Journal of Dental Research, vol. 87, no. 1, pp. 33-38. https://doi.org/10.1177/154405910808700105
Wittrant Y, Bhandari BS, Abboud H, Benson N, Woodruff K, MacDougall M et al. PDGF up-regulates CSF-1 gene transcription in ameloblast-like cells. Journal of Dental Research. 2008 Jan;87(1):33-38. https://doi.org/10.1177/154405910808700105
Wittrant, Y. ; Bhandari, B. Sriniketan ; Abboud, H. ; Benson, N. ; Woodruff, K. ; MacDougall, M. ; Abboud-Werner, S. / PDGF up-regulates CSF-1 gene transcription in ameloblast-like cells. In: Journal of Dental Research. 2008 ; Vol. 87, No. 1. pp. 33-38.
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