PDGF up-regulates CSF-1 gene transcription in ameloblast-like cells

Y. Wittrant, B. Sriniketan Bhandari, H. Abboud, N. Benson, K. Woodruff, M. MacDougall, S. Abboud-Werner

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Macrophage colony-stimulating factor (CSF-I) is a key regulatory cytokine for amelogenesis, and ameloblasts synthesize CSF-I. We hypothesized that PDGF stimulates DNA synthesis and regulates CSF-I in these cells. We determined the effect of PDGF on CSF-I expression using MEOE-3M ameloblasts as a model. By RT-PCR, MEOE-3M expressed PDGFRs and PDGF A- and B-chain mRNAs. PDGF-BB increased DNA synthesis and up-regulated CSF-I mRNA and protein in MEOE-3M. Cells transfected with CSF1 promoter deletion constructs were analyzed. A PDGF-responsive region between - 1.7 and - 0.795 kb, containing a consensus Pea3 binding motif, was identified. Electrophoretic mobility shift assay (EMSA) showed that PDGF-BB stimulated protein binding to this motif that was inhibited in the presence of anti-Pea3 antibody. Analysis of these data provides the first evidence that PDGFBB is a mitogen for MEOE-3M and increases CSF-I protein levels, predominantly by transcription. Elucidation of the cellular pathways that control CSF-1 expression may provide novel strategies for the regulation of enamel matrix formation.

Original languageEnglish (US)
Pages (from-to)33-38
Number of pages6
JournalJournal of dental research
Volume87
Issue number1
DOIs
StatePublished - Jan 2008

Keywords

  • Ameloblasts
  • Gene transcription
  • Macrophage colony-stimulating factor
  • Platelet-derived growth factor
  • Transcription factors

ASJC Scopus subject areas

  • Dentistry(all)

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