PDGF BB and bFGF stimulate DNA synthesis and upregulate CSF-1 and MCP-1 gene expression in dental follicle cells

S. Bsoul, Geza T Terezhalmy, H. Abboud, K. Woodruff, S. L. Abboud

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

CSF-1 and MCP-1, released by dental follicle cells, stimulate the influx of monocytes into the follicle sac and enhance the formation of osteoclasts that, in turn, resorb alveolar bone for the eruption pathway. PDGF and bFGF, released by cells adjacent to the follicle or by activated monocytes, are prime candidates that may regulate CSF-1 and MCP-1 gene expression. The present study demonstrates that PDGF and bFGF are mitosens for dental follicle cells and stimulate CSF-1 and MCP-1 mRNA, but with different time course kinetics. Peak induction of CSF-1 mRNA was observed at 6-8 h, white maximal MCP-1 induction was observed at 2 h. These findings suggest that MCP-1 is an early chemotactic signal for monocytes and that subsequent release of CSF-1 may act synergistically with MCP-1 to enhance monocyte influx. Further understanding of the molecular mechanisms by which cytokines regulate CSF-1 and MCP-1 may lead to more effective treatment regimens for disorders associated with abnormal tooth eruption.

Original languageEnglish (US)
Pages (from-to)459-465
Number of pages7
JournalArchives of Oral Biology
Volume48
Issue number6
DOIs
StatePublished - Jun 1 2003

Fingerprint

Dental Sac
Macrophage Colony-Stimulating Factor
Up-Regulation
Gene Expression
Monocytes
DNA
Tooth Eruption
Messenger RNA
Osteoclasts
platelet-derived growth factor BB
Cytokines
Bone and Bones

Keywords

  • bFGF
  • CSF-1
  • Dental follicle cells
  • Gene expression
  • MCP-1
  • PDGF

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

PDGF BB and bFGF stimulate DNA synthesis and upregulate CSF-1 and MCP-1 gene expression in dental follicle cells. / Bsoul, S.; Terezhalmy, Geza T; Abboud, H.; Woodruff, K.; Abboud, S. L.

In: Archives of Oral Biology, Vol. 48, No. 6, 01.06.2003, p. 459-465.

Research output: Contribution to journalArticle

Bsoul, S. ; Terezhalmy, Geza T ; Abboud, H. ; Woodruff, K. ; Abboud, S. L. / PDGF BB and bFGF stimulate DNA synthesis and upregulate CSF-1 and MCP-1 gene expression in dental follicle cells. In: Archives of Oral Biology. 2003 ; Vol. 48, No. 6. pp. 459-465.
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