PCR primers for human chromosomes: Reagents for the rapid analysis of somatic cell hybrids

S. Theune, J. Fung, S. Todd, A. Y. Sakaguchi, S. L. Naylor

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Rapid analysis of somatic cell hybrids can be facilitated by using the polymerase chain reaction (PCR) to assay for genes assigned to specific human chromosomes. We describe PCR primer pairs for genes on the short and long arms of the 22 autosomes and the X chromosome. Some of the primers were designed from the 3′ untranslated region of cDNA sequences, whereas others were derived from genomic sequence. Each primer set was tested for its specificity and mapped to a chromosome by screening a somatic cell hybrid panel. Two of the primer pairs (APOC2 and G6PD) detect CA dinucleotide repeat polymorphisms.

Original languageEnglish (US)
Pages (from-to)511-516
Number of pages6
JournalGenomics
Volume9
Issue number3
DOIs
StatePublished - Mar 1991

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'PCR primers for human chromosomes: Reagents for the rapid analysis of somatic cell hybrids'. Together they form a unique fingerprint.

  • Cite this