PCR primers for human chromosomes: Reagents for the rapid analysis of somatic cell hybrids

S. Theune; J. Fung; S. Todd; A. Y. Sakaguchi; S. L. Naylor

doi:10.1016/0888-7543(91)90418-E

PCR primers for human chromosomes: Reagents for the rapid analysis of somatic cell hybrids

S. Theune, J. Fung, S. Todd, A. Y. Sakaguchi, S. L. Naylor

Department of Cell Systems & Anatomy

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

Rapid analysis of somatic cell hybrids can be facilitated by using the polymerase chain reaction (PCR) to assay for genes assigned to specific human chromosomes. We describe PCR primer pairs for genes on the short and long arms of the 22 autosomes and the X chromosome. Some of the primers were designed from the 3′ untranslated region of cDNA sequences, whereas others were derived from genomic sequence. Each primer set was tested for its specificity and mapped to a chromosome by screening a somatic cell hybrid panel. Two of the primer pairs (APOC2 and G6PD) detect CA dinucleotide repeat polymorphisms.

Original language	English (US)
Pages (from-to)	511-516
Number of pages	6
Journal	Genomics
Volume	9
Issue number	3
DOIs	https://doi.org/10.1016/0888-7543(91)90418-E
State	Published - Mar 1991

ASJC Scopus subject areas

Genetics

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10.1016/0888-7543(91)90418-E

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title = "PCR primers for human chromosomes: Reagents for the rapid analysis of somatic cell hybrids",

abstract = "Rapid analysis of somatic cell hybrids can be facilitated by using the polymerase chain reaction (PCR) to assay for genes assigned to specific human chromosomes. We describe PCR primer pairs for genes on the short and long arms of the 22 autosomes and the X chromosome. Some of the primers were designed from the 3′ untranslated region of cDNA sequences, whereas others were derived from genomic sequence. Each primer set was tested for its specificity and mapped to a chromosome by screening a somatic cell hybrid panel. Two of the primer pairs (APOC2 and G6PD) detect CA dinucleotide repeat polymorphisms.",

author = "S. Theune and J. Fung and S. Todd and Sakaguchi, {A. Y.} and Naylor, {S. L.}",

note = "Funding Information: We thank Tom Shows, Norma Nowak, and Eric Green for cell hybrid DNA and helpful discussions in the preparation of this paper. We also thank Linda Howell for excellent assistance in the preparation of this paper. This work was supported by the National Institutes of Health (HG00315), the Mather Charitable Foundation, the Meadows Foundation, and the American Cancer Society (CD358A).",

year = "1991",

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doi = "10.1016/0888-7543(91)90418-E",

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T2 - Reagents for the rapid analysis of somatic cell hybrids

AU - Theune, S.

AU - Fung, J.

AU - Todd, S.

AU - Sakaguchi, A. Y.

AU - Naylor, S. L.

N1 - Funding Information: We thank Tom Shows, Norma Nowak, and Eric Green for cell hybrid DNA and helpful discussions in the preparation of this paper. We also thank Linda Howell for excellent assistance in the preparation of this paper. This work was supported by the National Institutes of Health (HG00315), the Mather Charitable Foundation, the Meadows Foundation, and the American Cancer Society (CD358A).

PY - 1991/3

Y1 - 1991/3

N2 - Rapid analysis of somatic cell hybrids can be facilitated by using the polymerase chain reaction (PCR) to assay for genes assigned to specific human chromosomes. We describe PCR primer pairs for genes on the short and long arms of the 22 autosomes and the X chromosome. Some of the primers were designed from the 3′ untranslated region of cDNA sequences, whereas others were derived from genomic sequence. Each primer set was tested for its specificity and mapped to a chromosome by screening a somatic cell hybrid panel. Two of the primer pairs (APOC2 and G6PD) detect CA dinucleotide repeat polymorphisms.

AB - Rapid analysis of somatic cell hybrids can be facilitated by using the polymerase chain reaction (PCR) to assay for genes assigned to specific human chromosomes. We describe PCR primer pairs for genes on the short and long arms of the 22 autosomes and the X chromosome. Some of the primers were designed from the 3′ untranslated region of cDNA sequences, whereas others were derived from genomic sequence. Each primer set was tested for its specificity and mapped to a chromosome by screening a somatic cell hybrid panel. Two of the primer pairs (APOC2 and G6PD) detect CA dinucleotide repeat polymorphisms.

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PCR primers for human chromosomes: Reagents for the rapid analysis of somatic cell hybrids

Abstract

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