PCR Cloning of an Orphan Homeobox Gene (PRH) Preferentially Expressed in Myeloid and Liver Cells

Robert A Hromas, Jerry Radich, Steven Collins

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Homeobox genes are members of a family of transcription factors that regulate tissue development in many different organisms. We set out to identify homeobox genes that might play a role in hematopoiesis. Using degenerate oligonucleotide primers corresponding to conserved homeobox sequences in the polymerase chain reaction (PCR), we cloned from the HL60 promyelocytic cell line a homeobox gene we now designate PRH. We mapped this gene, which was not homologous to any of the previously described homeobox genes, to chromosome 10, a region outside of the known genomic homeobox clusters. Northern blot hybridization indicates that PRH is preferentially expressed in myeloid and liver cells. The structure, chromosomal location, and pattern of expression of PRH indicate that it represents an orphan homeobox gene that may regulate the lineage development of certain hematopoietic cells.

Original languageEnglish (US)
Pages (from-to)976-983
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume195
Issue number2
DOIs
StatePublished - Sep 15 1993
Externally publishedYes

Fingerprint

Homeobox Genes
Cloning
Polymerase chain reaction
Myeloid Cells
Liver
Organism Cloning
Genes
Polymerase Chain Reaction
DNA Primers
Chromosomes, Human, Pair 10
Chromosomes
Conserved Sequence
HL-60 Cells
Hematopoiesis
Northern Blotting
Transcription Factors
Cells
Tissue
Cell Line

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

PCR Cloning of an Orphan Homeobox Gene (PRH) Preferentially Expressed in Myeloid and Liver Cells. / Hromas, Robert A; Radich, Jerry; Collins, Steven.

In: Biochemical and Biophysical Research Communications, Vol. 195, No. 2, 15.09.1993, p. 976-983.

Research output: Contribution to journalArticle

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