PAX1, a member of the paired box-containing class of developmental control genes, is mapped to human chromosome 20p11.2 by in Situ hybridization (ISH and FISH)

Susanne Schnittger, V. V.N. Gopal Rao, Urban Deutsch, Peter Gruss, Rudi Balling, Ingo Hansmann

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Pax-1, a member of a murine multigene family, belongs to the paired box-containing class of developmental control genes first identified in Drosophila. The Pax-1 gene encodes a sequence-specific DNA-binding protein with transcriptional activating properties and has been found to be mutated in the autosomal recessive mutation undulated (un) on mouse chromosome 2 with vertebral anomalies along the entire rostrocaudal axis. By radioactive in situ hybridization (ISH) using a fragment from the murine Pax-1 paired box that is almost identical to the respective sequences from the cognate human gene HuP48 and fluorescence in situ hybridization (FISH) using a complete mouse Pax-1 cDNA, we have assigned the human homologue of murine Pax-1, the PAX1 locus, to chromosome 20p. The map position of PAX1 after FISH (FL-pter value of 0.34 ± 0.04) corresponds to band p11.2. These results confirm the exceptional homology between human chromosome 20 and the distal segment of mouse chromosome 2, extending from bands F to G, and add PAX1 to the group of genes on 20p like PTPA, PRNP, SCG1, BMP2A, which are located in proximity on both chromosomes.

Original languageEnglish (US)
Pages (from-to)740-744
Number of pages5
JournalGenomics
Volume14
Issue number3
DOIs
StatePublished - Nov 1992
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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