Parathyroid hormone induces bone cell motility and loss of mature osteocyte phenotype through L-calcium channel dependent and independent mechanisms

Matthew Prideaux, Sarah L. Dallas, Ning Zhao, Erica D. Johnsrud, Patricia A. Veno, Dayong Guo, Yuji Mishina, Stephen E Harris, Lynda F. Bonewald

Research output: Contribution to journalArticle

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Abstract

Parathyroid Hormone (PTH) can exert both anabolic and catabolic effects on the skeleton, potentially through expression of the PTH type1 receptor (PTH1R), which is highly expressed in osteocytes. To determine the cellular and molecular mechanisms responsible, we examined the effects of PTH on osteoblast to osteocyte differentiation using primary osteocytes and the IDG-SW3 murine cell line, which differentiate from osteoblast to osteocyte-like cells in vitro and express GFP under control of the dentin matrix 1 (Dmp1) promoter. PTH treatment resulted in an increase in some osteoblast and early osteocyte markers and a decrease in mature osteocyte marker expression. The gene expression profile of PTH-treated Day 28 IDG-SW3 cells was similar to PTH treated primary osteocytes. PTH treatment induced striking changes in the morphology of the Dmp1-GFP positive cells in IDG-SW3 cultures and primary cells from Dmp1-GFP transgenic mice. The cells changed from a more dendritic to an elongated morphology and showed increased cell motility. E11/gp38 has been shown to be important for cell migration, however, deletion of the E11/gp38/podoplanin gene had no effect on PTH-induced motility. The effects of PTH on motility were reproduced using cAMP, but not with protein kinase A (PKA), exchange proteins activated by cAMP (Epac), protein kinase C (PKC) or phosphatidylinositol-4,5-bisphosphonate 3-kinase (Pi3K) agonists nor were they blocked by their antagonists. However, the effects of PTH were mediated through calcium signaling, specifically through L-type channels normally expressed in osteoblasts but decreased in osteocytes. PTH was shown to increase expression of this channel, but decrease the T-type channel that is normally more highly expressed in osteocytes. Inhibition of L-type calcium channel activity attenuated the effects of PTH on cell morphology and motility but did not prevent the downregulation of mature osteocyte marker expression. Taken together, these results show that PTH induces loss of the mature osteocyte phenotype and promotes the motility of these cells. These two effects are mediated through different mechanisms. The loss of phenotype effect is independent and the cell motility effect is dependent on calcium signaling.

Original languageEnglish (US)
Article numbere0125731
JournalPLoS One
Volume10
Issue number5
DOIs
StatePublished - May 5 2015

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Osteocytes
calcium channels
parathyroid hormone
Calcium Channels
Parathyroid Hormone
cell movement
Cell Movement
Bone
bones
Phenotype
phenotype
Bone and Bones
osteoblasts
Osteoblasts
Calcium Signaling
cells
Cells
Parathyroid Hormone Receptor Type 1
Calcium
Anabolic Agents

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Parathyroid hormone induces bone cell motility and loss of mature osteocyte phenotype through L-calcium channel dependent and independent mechanisms. / Prideaux, Matthew; Dallas, Sarah L.; Zhao, Ning; Johnsrud, Erica D.; Veno, Patricia A.; Guo, Dayong; Mishina, Yuji; Harris, Stephen E; Bonewald, Lynda F.

In: PLoS One, Vol. 10, No. 5, e0125731, 05.05.2015.

Research output: Contribution to journalArticle

Prideaux, M, Dallas, SL, Zhao, N, Johnsrud, ED, Veno, PA, Guo, D, Mishina, Y, Harris, SE & Bonewald, LF 2015, 'Parathyroid hormone induces bone cell motility and loss of mature osteocyte phenotype through L-calcium channel dependent and independent mechanisms', PLoS One, vol. 10, no. 5, e0125731. https://doi.org/10.1371/journal.pone.0125731
Prideaux, Matthew ; Dallas, Sarah L. ; Zhao, Ning ; Johnsrud, Erica D. ; Veno, Patricia A. ; Guo, Dayong ; Mishina, Yuji ; Harris, Stephen E ; Bonewald, Lynda F. / Parathyroid hormone induces bone cell motility and loss of mature osteocyte phenotype through L-calcium channel dependent and independent mechanisms. In: PLoS One. 2015 ; Vol. 10, No. 5.
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