Paraquat toxicity and oxidative damage

Reduction by Melatonin

Daniela Melchiorri, Russel J Reiter, Ewa Sewerynek, Masayuki Hara, Lidun Chen, Giuseppe Nisticò

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

The ability of melatonin to protect against paraquat-induced oxidative damage in rat lung, liver, and serum was examined. Changes in the levels of malondialdehyde (MDA) plus 4-hydroxyalkenals (4-HDA) and reduced and oxidized glutathione concentrations were measured. Paraquat (50 mg/kg) was injected i.p. into either Sprague-Dawley or Wistar rats with or without the co-administration of 5 mg/kg melatonin. Paraquat alone increased MDA + 4-HDA levels in serum and lungs of both rat strains, with these increases being abolished by melatonin co-treatment. Paraquat also decreased reduced glutathione levels and increased oxidized glutathione concentrations in lung and liver; these changes were negated by melatonin. The effect of melatonin on paraquat-induced mortality was also studied. Paraquat at a dose of 79 mg/kg was lethal for 50% of animals within 24 hr; when administered together with melatonin, the LD50 for paraquat increased to 251 mg/ kg.

Original languageEnglish (US)
Pages (from-to)1095-1099
Number of pages5
JournalBiochemical Pharmacology
Volume51
Issue number8
DOIs
StatePublished - Apr 26 1996

Fingerprint

Paraquat
Melatonin
Toxicity
Rats
Glutathione Disulfide
Malondialdehyde
Liver
Lung
Glutathione
Lethal Dose 50
Serum
Sprague Dawley Rats
Wistar Rats
Animals
Mortality

Keywords

  • Free radical-induced tissue damage
  • Melatonin as an antioxidant
  • Oxidative damage to lung and liver
  • Paraquat LD
  • Paraquat-induced lipid peroxidation
  • Reduced and oxidized glutathione

ASJC Scopus subject areas

  • Pharmacology

Cite this

Paraquat toxicity and oxidative damage : Reduction by Melatonin. / Melchiorri, Daniela; Reiter, Russel J; Sewerynek, Ewa; Hara, Masayuki; Chen, Lidun; Nisticò, Giuseppe.

In: Biochemical Pharmacology, Vol. 51, No. 8, 26.04.1996, p. 1095-1099.

Research output: Contribution to journalArticle

Melchiorri, D, Reiter, RJ, Sewerynek, E, Hara, M, Chen, L & Nisticò, G 1996, 'Paraquat toxicity and oxidative damage: Reduction by Melatonin', Biochemical Pharmacology, vol. 51, no. 8, pp. 1095-1099. https://doi.org/10.1016/0006-2952(96)00055-X
Melchiorri, Daniela ; Reiter, Russel J ; Sewerynek, Ewa ; Hara, Masayuki ; Chen, Lidun ; Nisticò, Giuseppe. / Paraquat toxicity and oxidative damage : Reduction by Melatonin. In: Biochemical Pharmacology. 1996 ; Vol. 51, No. 8. pp. 1095-1099.
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