Abstract
Parameters for detection of mutagenesis of 1,2-dimethylhydrazine (DMH) using a Bacillus subtilis microbial assay are defined. His+ and his*met* mutations were induced in B. subtilis TKJ6321 in the absence of rat liver S-9 Mix. This B. subtilis mutant showed a greater sensitivity to this chemical than did Salmonella typhimurium TA1535. Protein-binding studies with bovine serum albumin showed the capacity for DMH to bind non-specifically to protein. S-9 Mix from Aroclor® pretreated animals appeared to decrease the mutagenic response. Kinetics of mutagen stability were also shown, thereby demonstrating the need for more complete investigation of known chemical carcinogens which give negative results in the Ames bioassay alone.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 287-294 |
| Number of pages | 8 |
| Journal | Cancer Letters |
| Volume | 12 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1981 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Oncology
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Parameters for detection of mutagenesis of 1,2-dimethylhydrazine using an in vitro Bacillus subtilis system. / Wells, Barbara C.; Wargovich, Michael J; Felkner, I. Cecil.
In: Cancer Letters, Vol. 12, No. 4, 1981, p. 287-294.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Parameters for detection of mutagenesis of 1,2-dimethylhydrazine using an in vitro Bacillus subtilis system
AU - Wells, Barbara C.
AU - Wargovich, Michael J
AU - Felkner, I. Cecil
PY - 1981
Y1 - 1981
N2 - Parameters for detection of mutagenesis of 1,2-dimethylhydrazine (DMH) using a Bacillus subtilis microbial assay are defined. His+ and his*met* mutations were induced in B. subtilis TKJ6321 in the absence of rat liver S-9 Mix. This B. subtilis mutant showed a greater sensitivity to this chemical than did Salmonella typhimurium TA1535. Protein-binding studies with bovine serum albumin showed the capacity for DMH to bind non-specifically to protein. S-9 Mix from Aroclor® pretreated animals appeared to decrease the mutagenic response. Kinetics of mutagen stability were also shown, thereby demonstrating the need for more complete investigation of known chemical carcinogens which give negative results in the Ames bioassay alone.
AB - Parameters for detection of mutagenesis of 1,2-dimethylhydrazine (DMH) using a Bacillus subtilis microbial assay are defined. His+ and his*met* mutations were induced in B. subtilis TKJ6321 in the absence of rat liver S-9 Mix. This B. subtilis mutant showed a greater sensitivity to this chemical than did Salmonella typhimurium TA1535. Protein-binding studies with bovine serum albumin showed the capacity for DMH to bind non-specifically to protein. S-9 Mix from Aroclor® pretreated animals appeared to decrease the mutagenic response. Kinetics of mutagen stability were also shown, thereby demonstrating the need for more complete investigation of known chemical carcinogens which give negative results in the Ames bioassay alone.
UR - http://www.scopus.com/inward/record.url?scp=0019834160&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019834160&partnerID=8YFLogxK
U2 - 10.1016/0304-3835(81)90170-1
DO - 10.1016/0304-3835(81)90170-1
M3 - Article
C2 - 6796253
AN - SCOPUS:0019834160
VL - 12
SP - 287
EP - 294
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 4
ER -