Paramagnetism-Based Refinement Strategy for the Solution Structure of Human α-Parvalbumin

Irfan Baig, Ivano Bertini, Cristina Del Bianco, Yogesh K Gupta, Yong Min Lee, Claudio Luchinat, Alessandro Quattrone

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In the frame of a research aimed at the detailed structural characterization of human calcium-binding proteins of the EF-hand family, the solution structure of human α-parvalbumin has been solved by NMR and refined with the help of substitution of the Ca2+ ion in the EF site with the paramagnetic Dy3+ ion. A simple 1H- 15N HSQC spectrum allowed the NH assignments based on the properties of Dy3+. This allowed us to exploit pseudocontact shifts and residual dipolar couplings for solution structure refinement. The backbone and heavy atom RMSD are 0.55 ± 0.08 and 1.02 ± 0.08 Å, respectively, and decrease to 0.39 ± 0.05 and 0.90 ± 0.06 Å upon refinement with paramagnetism-based restraints. The RMSD for the metal itself in the EF site in the refined structure is 0.26 ± 0.12 Å. Backbone NH R1, R2, and NOE measured at two temperatures show the protein to be relatively rigid. The NH orientations are well determined by the paramagnetism-based restraints. This allows us to detect small but significant local structural differences with the orthologue protein from rat, whose X-ray structure is available at 2.0 Å resolution. All differences are related to local changes in the amino acidic composition.

Original languageEnglish (US)
Pages (from-to)5562-5573
Number of pages12
JournalBiochemistry
Volume43
Issue number18
DOIs
StatePublished - May 11 2004
Externally publishedYes

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Paramagnetism
Parvalbumins
Ions
EF Hand Motifs
Calcium-Binding Proteins
Rats
Proteins
Substitution reactions
Metals
Nuclear magnetic resonance
X-Rays
X rays
Atoms
Temperature
Chemical analysis
Research

ASJC Scopus subject areas

  • Biochemistry

Cite this

Baig, I., Bertini, I., Del Bianco, C., Gupta, Y. K., Lee, Y. M., Luchinat, C., & Quattrone, A. (2004). Paramagnetism-Based Refinement Strategy for the Solution Structure of Human α-Parvalbumin. Biochemistry, 43(18), 5562-5573. https://doi.org/10.1021/bi035879k

Paramagnetism-Based Refinement Strategy for the Solution Structure of Human α-Parvalbumin. / Baig, Irfan; Bertini, Ivano; Del Bianco, Cristina; Gupta, Yogesh K; Lee, Yong Min; Luchinat, Claudio; Quattrone, Alessandro.

In: Biochemistry, Vol. 43, No. 18, 11.05.2004, p. 5562-5573.

Research output: Contribution to journalArticle

Baig, I, Bertini, I, Del Bianco, C, Gupta, YK, Lee, YM, Luchinat, C & Quattrone, A 2004, 'Paramagnetism-Based Refinement Strategy for the Solution Structure of Human α-Parvalbumin', Biochemistry, vol. 43, no. 18, pp. 5562-5573. https://doi.org/10.1021/bi035879k
Baig, Irfan ; Bertini, Ivano ; Del Bianco, Cristina ; Gupta, Yogesh K ; Lee, Yong Min ; Luchinat, Claudio ; Quattrone, Alessandro. / Paramagnetism-Based Refinement Strategy for the Solution Structure of Human α-Parvalbumin. In: Biochemistry. 2004 ; Vol. 43, No. 18. pp. 5562-5573.
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