papel da IL-4 em modelo experimental de encefalite induzida pela inoculação intracraniana do herpes simplex vírus-1 (HSV-1)

Márcia Carvalho Vilela; Roberta Dayrell de Lima Campos; Daniel Santos Mansur; David Henrique Rodrigues; Norinne Lacerda-Queiroz; Graciela Kunrath Lima; Milene Alvarenga Rachid; Erna Geessien Kroon; Marco Antônio Campos; Antônio Lúcio Teixeira

doi:10.1590/S0004-282X2011000200019

papel da IL-4 em modelo experimental de encefalite induzida pela inoculação intracraniana do herpes simplex vírus-1 (HSV-1)

Translated title of the contribution: Role of IL-4 in an experimental model of encephalitis induced by intracranial inoculation of herpes simplex virus-1 (HSV-1)

Márcia Carvalho Vilela, Roberta Dayrell de Lima Campos, Daniel Santos Mansur, David Henrique Rodrigues, Norinne Lacerda-Queiroz, Graciela Kunrath Lima, Milene Alvarenga Rachid, Erna Geessien Kroon, Marco Antônio Campos, Antônio Lúcio Teixeira

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Herpes simplex virus-1 (HSV-1) is a pathogen that may cause severe encephalitis in humans. In this study, we aimed to investigate the role of interleukin-4 (IL-4) in a model of HSV-1 brain infection. IL-4 knockout (IL-4^-/-) and wild type (WT) C57BL/6 mice were inoculated with 10⁴ plaque-forming units of HSV-1 by the intracranial route. Histopathologic analysis revealed a distinct profile of infiltrating cells at 3 days postinfection (dpi). Infected WT mice presented mononuclear inflammatory cells while IL-4^-/- mice developed meningoencephalitis with predominance of neutrophils. IL-4-^/- mice had diminished leukocyte adhesion at 3 dpi when compared to infected WT animals in intravital microscopy study. Conversely no differences were found in cerebral levels of CXCL1, CXCL9, CCL3, CCL5 and TNF-α between WT and IL-4^-/- infected mice. IL-4 may play a role in the recruitment of cells into central nervous system in this acute model of severe encephalitis caused by HSV-1.

Translated title of the contribution	Role of IL-4 in an experimental model of encephalitis induced by intracranial inoculation of herpes simplex virus-1 (HSV-1)
Original language	Portuguese
Pages (from-to)	237-241
Number of pages	5
Journal	Arquivos de neuro-psiquiatria
Volume	69
Issue number	2 A
DOIs	https://doi.org/10.1590/S0004-282X2011000200019
State	Published - Apr 2011
Externally published	Yes

Keywords

Herpes simplex virus type 1
IL-4
Neuroinflammation

ASJC Scopus subject areas

Clinical Neurology
Biological Psychiatry

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10.1590/S0004-282X2011000200019

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Vilela, M. C., Campos, R. D. D. L., Mansur, D. S., Rodrigues, D. H., Lacerda-Queiroz, N., Lima, G. K., Rachid, M. A., Kroon, E. G., Campos, M. A., & Teixeira, A. L. (2011). papel da IL-4 em modelo experimental de encefalite induzida pela inoculação intracraniana do herpes simplex vírus-1 (HSV-1). Arquivos de neuro-psiquiatria, 69(2 A), 237-241. https://doi.org/10.1590/S0004-282X2011000200019

Vilela, MC, Campos, RDDL, Mansur, DS, Rodrigues, DH, Lacerda-Queiroz, N, Lima, GK, Rachid, MA, Kroon, EG, Campos, MA & Teixeira, AL 2011, 'papel da IL-4 em modelo experimental de encefalite induzida pela inoculação intracraniana do herpes simplex vírus-1 (HSV-1)', Arquivos de neuro-psiquiatria, vol. 69, no. 2 A, pp. 237-241. https://doi.org/10.1590/S0004-282X2011000200019

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abstract = "Herpes simplex virus-1 (HSV-1) is a pathogen that may cause severe encephalitis in humans. In this study, we aimed to investigate the role of interleukin-4 (IL-4) in a model of HSV-1 brain infection. IL-4 knockout (IL-4-/-) and wild type (WT) C57BL/6 mice were inoculated with 104 plaque-forming units of HSV-1 by the intracranial route. Histopathologic analysis revealed a distinct profile of infiltrating cells at 3 days postinfection (dpi). Infected WT mice presented mononuclear inflammatory cells while IL-4-/- mice developed meningoencephalitis with predominance of neutrophils. IL-4-/- mice had diminished leukocyte adhesion at 3 dpi when compared to infected WT animals in intravital microscopy study. Conversely no differences were found in cerebral levels of CXCL1, CXCL9, CCL3, CCL5 and TNF-α between WT and IL-4-/- infected mice. IL-4 may play a role in the recruitment of cells into central nervous system in this acute model of severe encephalitis caused by HSV-1.",

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papel da IL-4 em modelo experimental de encefalite induzida pela inoculação intracraniana do herpes simplex vírus-1 (HSV-1)

Abstract

Keywords

ASJC Scopus subject areas

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