P53 status influences regulation of HSPs and ribosomal proteins by PDTC and radiation

John S. Thompson, Reto Asmis, Judith Glass, Hua Liu, Colin Wilson, Brandy Nelson, Stephen A. Brown, Arnold J. Stromberg

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Pyrrolidine dithiocarbamate (PDTC) is a thiol-containing compound that can act under varying conditions as an anti-oxidant or pro-oxidant. Utilizing microarrays, we determined the effect of PDTC +/- ionizing radiation (IR) on the expression of heat shock protein (HSP) genes in isolated B6/129 wild-type (WT) and p53-/- spleen cells. Extremely significant microarrays demonstrated that PDTC, but not IR, markedly up-regulated the expression of the majority of detectable HSP genes in WT and many to a significantly greater degree in p53-/- deficient cells. Determination of the glutathione/glutathione disulfide ratio indicated that PDTC was acting as a pro-oxidant under these conditions. From these data we conclude that the clinical use of "antioxidants" with radiotherapy or chemotherapy must be very carefully based on knowledge of the p53 status of their intended normal and tumor target cells.

Original languageEnglish (US)
Pages (from-to)435-442
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume343
Issue number2
DOIs
StatePublished - May 5 2006

Keywords

  • Heat shock proteins
  • Ionizing radiation
  • Microarray
  • Pyrrolidine dithiocarbamate
  • p53

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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