P2X 3 is expressed by DRG neurons that terminate in inner lamina II

L. Vulchanova, M. S. Reidl, S. J. Shuster, L. S. Stone, Kenneth M Hargreaves, G. Buell, Supernant, R. A. North, R. Elde

Research output: Contribution to journalArticle

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Abstract

The P2X 3 receptor subunit, a member of the P2X family of ATP-gated ion channels, is almost exclusively localized in sensory neurons. In the present study, we sought to gain insight into the role of P2X 3 and P2X 3-containing neurons in sensory transmission, using immunohistochemical approaches. In rat dorsal root ganglia (DRG), P2X 3-immunoreactivity (-ir) was observed in small- and medium-sized neurons. Approximately 40% of DRG neuronal profiles in normal rats contained P2X 3-ir. In rats that had received neonatal capsaicin treatment, the number of P2X 3-positive neurons was decreased by approximately 70%. Analysis of the colocalization of P2X 3-ir with cytochemical markers of DRG neurons indicated that approximately 94% of the P2X 3-positive neuronal profiles were labelled by isolectin B 4 from Bandeiraea simplicifolia, while only 3% contained substance P-ir, and 7% contained somatostatin-ir. In dorsal horn of rat spinal cord, P2X 3-ir was observed in the inner portion of lamina II and was reduced subsequent to dorsal rhizotomy, as well as subsequent to neonatal capsaicin treatment. Finally, P2X 3-ir accumulated proximal to the site of sciatic nerve ligation, and was seen in nerve fibres in skin and corneal epithelium. In summary, our results suggest that P2X 3 is expressed by a functionally heterogeneous population of BSI-B 4-binding sensory neurons, and is transported into both central and peripheral processes of these neurons.

Original languageEnglish (US)
Pages (from-to)3470-3478
Number of pages9
JournalEuropean Journal of Neuroscience
Volume10
Issue number11
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Substantia Gelatinosa
Spinal Ganglia
Sensory Receptor Cells
Neurons
Capsaicin
Rhizotomy
Corneal Epithelium
Sciatic Nerve
Substance P
Somatostatin
Ion Channels
Nerve Fibers
Lectins
Ligation
Adenosine Triphosphate
Skin
Population

Keywords

  • ATP
  • FRAP
  • IB4
  • Isolectin B
  • P2X
  • Rat
  • Sensory neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Vulchanova, L., Reidl, M. S., Shuster, S. J., Stone, L. S., Hargreaves, K. M., Buell, G., ... Elde, R. (1998). P2X 3 is expressed by DRG neurons that terminate in inner lamina II. European Journal of Neuroscience, 10(11), 3470-3478. https://doi.org/10.1046/j.1460-9568.1998.00355.x

P2X 3 is expressed by DRG neurons that terminate in inner lamina II. / Vulchanova, L.; Reidl, M. S.; Shuster, S. J.; Stone, L. S.; Hargreaves, Kenneth M; Buell, G.; Supernant; North, R. A.; Elde, R.

In: European Journal of Neuroscience, Vol. 10, No. 11, 1998, p. 3470-3478.

Research output: Contribution to journalArticle

Vulchanova, L, Reidl, MS, Shuster, SJ, Stone, LS, Hargreaves, KM, Buell, G, Supernant, North, RA & Elde, R 1998, 'P2X 3 is expressed by DRG neurons that terminate in inner lamina II', European Journal of Neuroscience, vol. 10, no. 11, pp. 3470-3478. https://doi.org/10.1046/j.1460-9568.1998.00355.x
Vulchanova, L. ; Reidl, M. S. ; Shuster, S. J. ; Stone, L. S. ; Hargreaves, Kenneth M ; Buell, G. ; Supernant ; North, R. A. ; Elde, R. / P2X 3 is expressed by DRG neurons that terminate in inner lamina II. In: European Journal of Neuroscience. 1998 ; Vol. 10, No. 11. pp. 3470-3478.
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abstract = "The P2X 3 receptor subunit, a member of the P2X family of ATP-gated ion channels, is almost exclusively localized in sensory neurons. In the present study, we sought to gain insight into the role of P2X 3 and P2X 3-containing neurons in sensory transmission, using immunohistochemical approaches. In rat dorsal root ganglia (DRG), P2X 3-immunoreactivity (-ir) was observed in small- and medium-sized neurons. Approximately 40{\%} of DRG neuronal profiles in normal rats contained P2X 3-ir. In rats that had received neonatal capsaicin treatment, the number of P2X 3-positive neurons was decreased by approximately 70{\%}. Analysis of the colocalization of P2X 3-ir with cytochemical markers of DRG neurons indicated that approximately 94{\%} of the P2X 3-positive neuronal profiles were labelled by isolectin B 4 from Bandeiraea simplicifolia, while only 3{\%} contained substance P-ir, and 7{\%} contained somatostatin-ir. In dorsal horn of rat spinal cord, P2X 3-ir was observed in the inner portion of lamina II and was reduced subsequent to dorsal rhizotomy, as well as subsequent to neonatal capsaicin treatment. Finally, P2X 3-ir accumulated proximal to the site of sciatic nerve ligation, and was seen in nerve fibres in skin and corneal epithelium. In summary, our results suggest that P2X 3 is expressed by a functionally heterogeneous population of BSI-B 4-binding sensory neurons, and is transported into both central and peripheral processes of these neurons.",
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AU - Vulchanova, L.

AU - Reidl, M. S.

AU - Shuster, S. J.

AU - Stone, L. S.

AU - Hargreaves, Kenneth M

AU - Buell, G.

AU - Supernant,

AU - North, R. A.

AU - Elde, R.

PY - 1998

Y1 - 1998

N2 - The P2X 3 receptor subunit, a member of the P2X family of ATP-gated ion channels, is almost exclusively localized in sensory neurons. In the present study, we sought to gain insight into the role of P2X 3 and P2X 3-containing neurons in sensory transmission, using immunohistochemical approaches. In rat dorsal root ganglia (DRG), P2X 3-immunoreactivity (-ir) was observed in small- and medium-sized neurons. Approximately 40% of DRG neuronal profiles in normal rats contained P2X 3-ir. In rats that had received neonatal capsaicin treatment, the number of P2X 3-positive neurons was decreased by approximately 70%. Analysis of the colocalization of P2X 3-ir with cytochemical markers of DRG neurons indicated that approximately 94% of the P2X 3-positive neuronal profiles were labelled by isolectin B 4 from Bandeiraea simplicifolia, while only 3% contained substance P-ir, and 7% contained somatostatin-ir. In dorsal horn of rat spinal cord, P2X 3-ir was observed in the inner portion of lamina II and was reduced subsequent to dorsal rhizotomy, as well as subsequent to neonatal capsaicin treatment. Finally, P2X 3-ir accumulated proximal to the site of sciatic nerve ligation, and was seen in nerve fibres in skin and corneal epithelium. In summary, our results suggest that P2X 3 is expressed by a functionally heterogeneous population of BSI-B 4-binding sensory neurons, and is transported into both central and peripheral processes of these neurons.

AB - The P2X 3 receptor subunit, a member of the P2X family of ATP-gated ion channels, is almost exclusively localized in sensory neurons. In the present study, we sought to gain insight into the role of P2X 3 and P2X 3-containing neurons in sensory transmission, using immunohistochemical approaches. In rat dorsal root ganglia (DRG), P2X 3-immunoreactivity (-ir) was observed in small- and medium-sized neurons. Approximately 40% of DRG neuronal profiles in normal rats contained P2X 3-ir. In rats that had received neonatal capsaicin treatment, the number of P2X 3-positive neurons was decreased by approximately 70%. Analysis of the colocalization of P2X 3-ir with cytochemical markers of DRG neurons indicated that approximately 94% of the P2X 3-positive neuronal profiles were labelled by isolectin B 4 from Bandeiraea simplicifolia, while only 3% contained substance P-ir, and 7% contained somatostatin-ir. In dorsal horn of rat spinal cord, P2X 3-ir was observed in the inner portion of lamina II and was reduced subsequent to dorsal rhizotomy, as well as subsequent to neonatal capsaicin treatment. Finally, P2X 3-ir accumulated proximal to the site of sciatic nerve ligation, and was seen in nerve fibres in skin and corneal epithelium. In summary, our results suggest that P2X 3 is expressed by a functionally heterogeneous population of BSI-B 4-binding sensory neurons, and is transported into both central and peripheral processes of these neurons.

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