P2X 3 is expressed by DRG neurons that terminate in inner lamina II

L. Vulchanova, M. S. Reidl, S. J. Shuster, L. S. Stone, K. M. Hargreaves, G. Buell, Supernant, R. A. North, R. Elde

Research output: Contribution to journalArticlepeer-review

290 Scopus citations

Abstract

The P2X 3 receptor subunit, a member of the P2X family of ATP-gated ion channels, is almost exclusively localized in sensory neurons. In the present study, we sought to gain insight into the role of P2X 3 and P2X 3-containing neurons in sensory transmission, using immunohistochemical approaches. In rat dorsal root ganglia (DRG), P2X 3-immunoreactivity (-ir) was observed in small- and medium-sized neurons. Approximately 40% of DRG neuronal profiles in normal rats contained P2X 3-ir. In rats that had received neonatal capsaicin treatment, the number of P2X 3-positive neurons was decreased by approximately 70%. Analysis of the colocalization of P2X 3-ir with cytochemical markers of DRG neurons indicated that approximately 94% of the P2X 3-positive neuronal profiles were labelled by isolectin B 4 from Bandeiraea simplicifolia, while only 3% contained substance P-ir, and 7% contained somatostatin-ir. In dorsal horn of rat spinal cord, P2X 3-ir was observed in the inner portion of lamina II and was reduced subsequent to dorsal rhizotomy, as well as subsequent to neonatal capsaicin treatment. Finally, P2X 3-ir accumulated proximal to the site of sciatic nerve ligation, and was seen in nerve fibres in skin and corneal epithelium. In summary, our results suggest that P2X 3 is expressed by a functionally heterogeneous population of BSI-B 4-binding sensory neurons, and is transported into both central and peripheral processes of these neurons.

Original languageEnglish (US)
Pages (from-to)3470-3478
Number of pages9
JournalEuropean Journal of Neuroscience
Volume10
Issue number11
DOIs
StatePublished - Dec 1 1998
Externally publishedYes

Keywords

  • ATP
  • FRAP
  • IB4
  • Isolectin B
  • P2X
  • Rat
  • Sensory neurons

ASJC Scopus subject areas

  • Neuroscience(all)

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