Overexpression of superoxide dismutase and catalase in immortalized neural cells: Toxic effects of hydrogen peroxide

Hema Mann, Michael T. McCoy, Jayanthi Subramaniam, Holly Van Remmen, Jean Lud Cadet

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Hydrogen peroxide (H2O2) is a known toxicant which causes its damage via the production of hydroxyl radicals. It has been reported to cause both necrotic and apoptotic cell death. The present study was undertaken to evaluate the mode of H2O2-induced cell death and to assess if overexpression of catalase could protect against its toxicity H2O2 causes cell death of immortalized CSM 14.1 neural cells in a dose-dependent manner H2O2-induced death was associated with DNA laddering as shown by agarose gel electrophoresis. Stable overexpression of catalase by transfection of a vector containing human cDNA into these cells markedly attenuated H2O2- induced toxic effects. Transfection of a vector containing a SOD cDNA afforded no protection. These results indicate that H2O2 can lead to the activation of endonuclease enzyme that breaks DNA into oligosomes. These cells which overexpress catalase or SOD will help to determine the specific role of H2O2 or O2/- in the deleterious effects of a number of toxins.

Original languageEnglish (US)
Pages (from-to)163-168
Number of pages6
JournalBrain Research
Volume770
Issue number1-2
DOIs
StatePublished - Oct 3 1997

Keywords

  • Catalase
  • Cytotoxicity
  • Hydrogen peroxide
  • Superoxide dismutase

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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