Overexpression of protein kinase C βI in a murine keratinocyte cell line produces effects on cellular growth, morphology and differentiation

Kevin R. O'Driscoll, Patrick V. Madden, Kim M. Christiansen, Aurora Viage, Thomas J. Slaga, Dorianno Fabbro, C. Thomas Powell, I. Bernard Weinstein

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The present study demonstrates that the murine keratinocyte cell line 3PC expresses the Ca2+-insensitive isoforms of protein kinase C (PKC) δ, ε{lunate}, ζ and (at both the mRNA and protein levels), but does not express the Ca2+-sensitive PKC isoforms α, β or γ. Recombinant retroviral gene transduction was used to develop derivatives of this cell line that stably express high levels of 1 PKCβI-related transcripts and proteins, and have increased levels of Ca2+-stimulated PKC enzyme activity. Functional overexpression of the PKCβI isoform in 3PC cells enhances both 12-O-tetradecanoyl phorbol-13-acetate-induced growth inhibition, and Ca2+-induced morphologic differentiation.

Original languageEnglish (US)
Pages (from-to)249-259
Number of pages11
JournalCancer Letters
Volume83
Issue number1-2
DOIs
StatePublished - Aug 15 1994
Externally publishedYes

Keywords

  • 12-O-Tetradecanoyl phorbol-13-acetate
  • Epidermal differentiation
  • Multistage carcinogenesis
  • Murine keratinocytes
  • Protein kinase C isoforms
  • Tumor promotion

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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