TY - JOUR
T1 - Overexpression of protein kinase C βI in a murine keratinocyte cell line produces effects on cellular growth, morphology and differentiation
AU - O'Driscoll, Kevin R.
AU - Madden, Patrick V.
AU - Christiansen, Kim M.
AU - Viage, Aurora
AU - Slaga, Thomas J.
AU - Fabbro, Dorianno
AU - Powell, C. Thomas
AU - Weinstein, I. Bernard
N1 - Funding Information:
The authors wish to thank Drs Y. Arita, T. Bowden. A. Dlugosz, S. Jaken, J. Knopf, M. Mat-sui, K.-M. Tchou-Wong, M. Ueffing, B.S. Warren, and S.H. Yuspa for providing valuable reagentso r discussions. This work was funded in part by a National Cancer Institute grant, CA02656 (to I.B. Weinstein); by an award from
Funding Information:
the Lucille Markey Charitable Trust (to I.B. Weinstein); and by a National Research Service Award, CA09529-05 postdoctoral fellowship (to K.R. O’Driscoll).
PY - 1994/8/15
Y1 - 1994/8/15
N2 - The present study demonstrates that the murine keratinocyte cell line 3PC expresses the Ca2+-insensitive isoforms of protein kinase C (PKC) δ, ε{lunate}, ζ and (at both the mRNA and protein levels), but does not express the Ca2+-sensitive PKC isoforms α, β or γ. Recombinant retroviral gene transduction was used to develop derivatives of this cell line that stably express high levels of 1 PKCβI-related transcripts and proteins, and have increased levels of Ca2+-stimulated PKC enzyme activity. Functional overexpression of the PKCβI isoform in 3PC cells enhances both 12-O-tetradecanoyl phorbol-13-acetate-induced growth inhibition, and Ca2+-induced morphologic differentiation.
AB - The present study demonstrates that the murine keratinocyte cell line 3PC expresses the Ca2+-insensitive isoforms of protein kinase C (PKC) δ, ε{lunate}, ζ and (at both the mRNA and protein levels), but does not express the Ca2+-sensitive PKC isoforms α, β or γ. Recombinant retroviral gene transduction was used to develop derivatives of this cell line that stably express high levels of 1 PKCβI-related transcripts and proteins, and have increased levels of Ca2+-stimulated PKC enzyme activity. Functional overexpression of the PKCβI isoform in 3PC cells enhances both 12-O-tetradecanoyl phorbol-13-acetate-induced growth inhibition, and Ca2+-induced morphologic differentiation.
KW - 12-O-Tetradecanoyl phorbol-13-acetate
KW - Epidermal differentiation
KW - Multistage carcinogenesis
KW - Murine keratinocytes
KW - Protein kinase C isoforms
KW - Tumor promotion
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U2 - 10.1016/0304-3835(94)90327-1
DO - 10.1016/0304-3835(94)90327-1
M3 - Article
C2 - 8062223
AN - SCOPUS:0028128762
SN - 0304-3835
VL - 83
SP - 249
EP - 259
JO - Cancer Letters
JF - Cancer Letters
IS - 1-2
ER -