TY - JOUR
T1 - Overexpression of extracellular sucrase (SacC) of Zymomonas mobilis in Escherichia coli
AU - Kannan, Rengasamy
AU - Pitchaimani, Kandasamy
AU - Gunasekaran, Paramasamy
AU - Aït-abdelkader, nadra
AU - Baratti, Jacques
PY - 1995/11/1
Y1 - 1995/11/1
N2 - The extracellular sucrase (SacC) gene of Zymomonas mobilis was overexpressed in Escherichia coli BL21 using the T7 polymerase expression system. A low cell density induction method was designed to have maximum expression, and the conditions (IPTG concentration, ampicillin addition) were optimised to overexpress to the level of more than 60% of the total cellular protein representing SacC protein.
AB - The extracellular sucrase (SacC) gene of Zymomonas mobilis was overexpressed in Escherichia coli BL21 using the T7 polymerase expression system. A low cell density induction method was designed to have maximum expression, and the conditions (IPTG concentration, ampicillin addition) were optimised to overexpress to the level of more than 60% of the total cellular protein representing SacC protein.
KW - Escherichia coli
KW - Overexpression
KW - Sucrase
KW - Zymomonas mobilis
UR - http://www.scopus.com/inward/record.url?scp=0028807057&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028807057&partnerID=8YFLogxK
U2 - 10.1016/0378-1097(95)00327-2
DO - 10.1016/0378-1097(95)00327-2
M3 - Article
C2 - 8566709
AN - SCOPUS:0028807057
SN - 0378-1097
VL - 133
SP - 29
EP - 33
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1-2
ER -