Overexpression of extracellular sucrase (SacC) of Zymomonas mobilis in Escherichia coli

Rengasamy Kannan, Kandasamy Pitchaimani, Paramasamy Gunasekaran, nadra Aït-abdelkader, Jacques Baratti

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The extracellular sucrase (SacC) gene of Zymomonas mobilis was overexpressed in Escherichia coli BL21 using the T7 polymerase expression system. A low cell density induction method was designed to have maximum expression, and the conditions (IPTG concentration, ampicillin addition) were optimised to overexpress to the level of more than 60% of the total cellular protein representing SacC protein.

Original languageEnglish (US)
Pages (from-to)29-33
Number of pages5
JournalFEMS Microbiology Letters
Volume133
Issue number1-2
DOIs
StatePublished - Nov 1 1995

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Keywords

  • Escherichia coli
  • Overexpression
  • Sucrase
  • Zymomonas mobilis

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Kannan, R., Pitchaimani, K., Gunasekaran, P., Aït-abdelkader, N., & Baratti, J. (1995). Overexpression of extracellular sucrase (SacC) of Zymomonas mobilis in Escherichia coli. FEMS Microbiology Letters, 133(1-2), 29-33. https://doi.org/10.1016/0378-1097(95)00327-2