Abstract
Bacteroides nodosus is the essential causative agent of ovine footrot. It produces extracellular proteases which involved in pathogenesis of footrot. In this paper, we report the subcloning of Bacteroides nodosus protease, its overexpression in E. coli and its N-terminal polypeptide sequence. The subclone library was constructed in E. coli using SphI digested original clone (15 kb) and plasmid PTZ18R and screened using immunological assay. The expression was observed using SDS-PAGE. The subcloned DNA fragment was then cut with Sau3AI, cloned into pKK232-8 vector to perform promoter isolation and analysis. The promoter strength was determined using spectrophotometric assay.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 433-437 |
| Number of pages | 5 |
| Journal | Wei sheng wu xue bao = Acta microbiologica Sinica |
| Volume | 31 |
| Issue number | 6 |
| State | Published - Dec 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
Overexpression of an extracellular Bacteroides nodosus protease in E. coli and isolation and characterization of its promoter. / Gong, Y.; Moses, E.
In: Wei sheng wu xue bao = Acta microbiologica Sinica, Vol. 31, No. 6, 12.1991, p. 433-437.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Overexpression of an extracellular Bacteroides nodosus protease in E. coli and isolation and characterization of its promoter
AU - Gong, Y.
AU - Moses, E.
PY - 1991/12
Y1 - 1991/12
N2 - Bacteroides nodosus is the essential causative agent of ovine footrot. It produces extracellular proteases which involved in pathogenesis of footrot. In this paper, we report the subcloning of Bacteroides nodosus protease, its overexpression in E. coli and its N-terminal polypeptide sequence. The subclone library was constructed in E. coli using SphI digested original clone (15 kb) and plasmid PTZ18R and screened using immunological assay. The expression was observed using SDS-PAGE. The subcloned DNA fragment was then cut with Sau3AI, cloned into pKK232-8 vector to perform promoter isolation and analysis. The promoter strength was determined using spectrophotometric assay.
AB - Bacteroides nodosus is the essential causative agent of ovine footrot. It produces extracellular proteases which involved in pathogenesis of footrot. In this paper, we report the subcloning of Bacteroides nodosus protease, its overexpression in E. coli and its N-terminal polypeptide sequence. The subclone library was constructed in E. coli using SphI digested original clone (15 kb) and plasmid PTZ18R and screened using immunological assay. The expression was observed using SDS-PAGE. The subcloned DNA fragment was then cut with Sau3AI, cloned into pKK232-8 vector to perform promoter isolation and analysis. The promoter strength was determined using spectrophotometric assay.
UR - http://www.scopus.com/inward/record.url?scp=0026310732&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026310732&partnerID=8YFLogxK
M3 - Article
C2 - 1814046
AN - SCOPUS:0026310732
VL - 31
SP - 433
EP - 437
JO - Wei sheng wu xue bao = Acta microbiologica Sinica
JF - Wei sheng wu xue bao = Acta microbiologica Sinica
SN - 0001-6209
IS - 6
ER -