Overexpression of an extracellular Bacteroides nodosus protease in E. coli and isolation and characterization of its promoter

Y. Gong, E. Moses

    Research output: Contribution to journalArticle

    Abstract

    Bacteroides nodosus is the essential causative agent of ovine footrot. It produces extracellular proteases which involved in pathogenesis of footrot. In this paper, we report the subcloning of Bacteroides nodosus protease, its overexpression in E. coli and its N-terminal polypeptide sequence. The subclone library was constructed in E. coli using SphI digested original clone (15 kb) and plasmid PTZ18R and screened using immunological assay. The expression was observed using SDS-PAGE. The subcloned DNA fragment was then cut with Sau3AI, cloned into pKK232-8 vector to perform promoter isolation and analysis. The promoter strength was determined using spectrophotometric assay.

    Original languageEnglish (US)
    Pages (from-to)433-437
    Number of pages5
    JournalWei sheng wu xue bao = Acta microbiologica Sinica
    Volume31
    Issue number6
    StatePublished - Dec 1991

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    ASJC Scopus subject areas

    • Medicine(all)

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