Overexpression of an extracellular Bacteroides nodosus protease in E. coli and isolation and characterization of its promoter

Y. Gong, E. Moses

Research output: Contribution to journalArticle

Abstract

Bacteroides nodosus is the essential causative agent of ovine footrot. It produces extracellular proteases which involved in pathogenesis of footrot. In this paper, we report the subcloning of Bacteroides nodosus protease, its overexpression in E. coli and its N-terminal polypeptide sequence. The subclone library was constructed in E. coli using SphI digested original clone (15 kb) and plasmid PTZ18R and screened using immunological assay. The expression was observed using SDS-PAGE. The subcloned DNA fragment was then cut with Sau3AI, cloned into pKK232-8 vector to perform promoter isolation and analysis. The promoter strength was determined using spectrophotometric assay.

Original languageEnglish (US)
Pages (from-to)433-437
Number of pages5
JournalWei sheng wu xue bao = Acta microbiologica Sinica
Volume31
Issue number6
StatePublished - Dec 1991
Externally publishedYes

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Dichelobacter nodosus
Peptide Hydrolases
Escherichia coli
Libraries
Polyacrylamide Gel Electrophoresis
Sheep
Plasmids
Clone Cells
Peptides
DNA
protease E

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Overexpression of an extracellular Bacteroides nodosus protease in E. coli and isolation and characterization of its promoter. / Gong, Y.; Moses, E.

In: Wei sheng wu xue bao = Acta microbiologica Sinica, Vol. 31, No. 6, 12.1991, p. 433-437.

Research output: Contribution to journalArticle

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