Ovariectomy-induced bone loss and the hematopoietic system

Dike N. Kalu, Elena Salerno, Chung Ching Liu, Fabrizio Ferarro, Bahram N. Arjmandi, Mohammed A. Salih

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

To investigate the relationship of the hematopoietic system to the loss of bone due to ovarian hormone deficiency, we examined the effects of ovariectomy and estrogen administration on the thymus, spleen and the bone marrow, and on the proliferation of marrow progenitors of osteoclasts. We also assessed the effects of daily administration of interleukin-1 receptor antagonist (IL-lra) on bone loss due to ovarian hormone deficiency. Ovariectomy resulted in decreased cancellous bone volume, increased trabecular osteoblast and osteoclast numbers, and increased serum alkaline phosphatase levels that were prevented by 17β-estradiol treatment. Thymus weight, spleen weight, thymus and spleen lymphocytes, and bone marrow monocytes and lymphocytes also increased significantly following ovariectomy, and the increases were suppressed by 17β-estradiol. Ovariectomy, in addition, caused a 4-fold increase in the number of tartrate resistant acid phosphatase (TRAP)-positive multinucleated cells formed in cultures of marrow cells and the increase was partially inhibited by 17β-estradiol. IL-1ra administration did not prevent the bone loss due to ovariectomy. Our findings indicate that ovariectomy-induced bone loss in the rat is accompanied by marked changes in the hematopoietic system, and that these changes are modulated by estrogen administration. In spite of the negative finding with IL-1ra, the nature of the involvement of the hematopoietic system in the pathogenesis of bone loss due to ovarian hormone deficiency merits continued exploration.

Original languageEnglish (US)
Pages (from-to)145-161
Number of pages17
JournalBone and Mineral
Volume23
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

Keywords

  • Bone loss
  • Estradiol
  • Hematopoietic system
  • IL-1ra
  • Ovariectomy

ASJC Scopus subject areas

  • Surgery
  • Biochemistry
  • Endocrinology

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