Abstract
Bacterial peri-implant biofilms, and the chemotherapeutics for their removal alter titanium surface cytocompatibility. In this study we aimed to assess the adjunctive use of an osteostimulative biomaterial utilizing a peri-implantitis model under the hypothesis that it will increase cell migration towards treated titanium surfaces. Acid-etched titanium surfaces were inoculated with a multi-species biofilm model and treated with 1.5% NaOCl in a previously characterized in vitro peri-implantitis model. Cell migration of MG63 cells towards the treated titanium surface (CTRL) was significantly reduced following inoculation with biofilm and chemotherapeutic treatment as compared to sterile controls. Addition of a tricalcium phosphate biomaterial (TCP) as a control for Ca+2 had a small non-significant effect, while BG significantly increased MG63 chemotaxis to titanium to levels comparable to sterile (STE). Similarly, cell viability at 5 days was increased in BG and TCP as compared to CTRL. SEM imaging confirmed the improved cytocompatibility of BG and TCP surfaces as compared to CTRL. Osteostimulative BG exhibited a strong chemotactic effect to osteoblasts, which was stronger than what was expected due to the chemotactic effect of Ca+2 alone (TCP). In addition, substantially increased cell attachment and viability was found on treated implant surfaces as compared to CTRL.
Original language | English (US) |
---|---|
Pages (from-to) | 2645-2652 |
Number of pages | 8 |
Journal | Journal of Biomedical Materials Research - Part B Applied Biomaterials |
Volume | 106 |
Issue number | 7 |
DOIs | |
State | Published - Oct 2018 |
Externally published | Yes |
Keywords
- bioactive glass
- calcium phosphosilicate
- dental
- osteoblast
- peri-implantitis
- titanium
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering