Abstract
Osteogenic protein-1 (OP-1 or BMP-7) stimulates new bone formation in vivo and induces cell proliferation and differentiation of osteoblasts in vitro. In the present study, we examined effects of OP-1 on the expression of vascular endothelial growth factor (VEGF) in primary cultures of fetal rat calvaria (FRC) cells. OP-1 increased the steady-state level of VEGF mRNA by about 3-fold in an OP-1 concentration- and time-dependent manner. The increase in VEGF mRNA level depended on transcription and was sensitive to cell replication. The VEGF mRNA stability was unaffected. The mRNA levels for both types of VEGF receptors, Flk-1 and Flt-1 were low but detectable in FRC cells by RT-PCR and were not changed by OP-1. Inhibition of VEGF synthesis and function by antisense oligonucleotide and by suramin, respectively arrested the OP-1-induced alkaline phosphatase activity and mineralized bone nodule formation. Together with published studies of VEGF on vascular endothelial cells which are usually found in close proximity to osteoblastic cells in vivo, these results suggest that VEGF participates in the OP-1- induced osteogenesis by taking part in bone cell differentiation and by promoting angiogenesis at the side of bone formation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 113-124 |
| Number of pages | 12 |
| Journal | Molecular and Cellular Endocrinology |
| Volume | 153 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Jul 20 1999 |
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Keywords
- BMP
- Osteoblast
- Osteogenic protein-1
- VEGF expression
ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
Cite this
Osteogenic protein-1 increases gene expression of vascular endothelial growth factor in primary cultures of fetal rat calvaria cells. / Yeh, Lee Chuan C; Lee, John C.
In: Molecular and Cellular Endocrinology, Vol. 153, No. 1-2, 20.07.1999, p. 113-124.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Osteogenic protein-1 increases gene expression of vascular endothelial growth factor in primary cultures of fetal rat calvaria cells
AU - Yeh, Lee Chuan C
AU - Lee, John C.
PY - 1999/7/20
Y1 - 1999/7/20
N2 - Osteogenic protein-1 (OP-1 or BMP-7) stimulates new bone formation in vivo and induces cell proliferation and differentiation of osteoblasts in vitro. In the present study, we examined effects of OP-1 on the expression of vascular endothelial growth factor (VEGF) in primary cultures of fetal rat calvaria (FRC) cells. OP-1 increased the steady-state level of VEGF mRNA by about 3-fold in an OP-1 concentration- and time-dependent manner. The increase in VEGF mRNA level depended on transcription and was sensitive to cell replication. The VEGF mRNA stability was unaffected. The mRNA levels for both types of VEGF receptors, Flk-1 and Flt-1 were low but detectable in FRC cells by RT-PCR and were not changed by OP-1. Inhibition of VEGF synthesis and function by antisense oligonucleotide and by suramin, respectively arrested the OP-1-induced alkaline phosphatase activity and mineralized bone nodule formation. Together with published studies of VEGF on vascular endothelial cells which are usually found in close proximity to osteoblastic cells in vivo, these results suggest that VEGF participates in the OP-1- induced osteogenesis by taking part in bone cell differentiation and by promoting angiogenesis at the side of bone formation.
AB - Osteogenic protein-1 (OP-1 or BMP-7) stimulates new bone formation in vivo and induces cell proliferation and differentiation of osteoblasts in vitro. In the present study, we examined effects of OP-1 on the expression of vascular endothelial growth factor (VEGF) in primary cultures of fetal rat calvaria (FRC) cells. OP-1 increased the steady-state level of VEGF mRNA by about 3-fold in an OP-1 concentration- and time-dependent manner. The increase in VEGF mRNA level depended on transcription and was sensitive to cell replication. The VEGF mRNA stability was unaffected. The mRNA levels for both types of VEGF receptors, Flk-1 and Flt-1 were low but detectable in FRC cells by RT-PCR and were not changed by OP-1. Inhibition of VEGF synthesis and function by antisense oligonucleotide and by suramin, respectively arrested the OP-1-induced alkaline phosphatase activity and mineralized bone nodule formation. Together with published studies of VEGF on vascular endothelial cells which are usually found in close proximity to osteoblastic cells in vivo, these results suggest that VEGF participates in the OP-1- induced osteogenesis by taking part in bone cell differentiation and by promoting angiogenesis at the side of bone formation.
KW - BMP
KW - Osteoblast
KW - Osteogenic protein-1
KW - VEGF expression
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UR - http://www.scopus.com/inward/citedby.url?scp=0033587653&partnerID=8YFLogxK
U2 - 10.1016/S0303-7207(99)00076-3
DO - 10.1016/S0303-7207(99)00076-3
M3 - Article
C2 - 10459859
AN - SCOPUS:0033587653
VL - 153
SP - 113
EP - 124
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 1-2
ER -