Osteogenic protein-1 increases gene expression of vascular endothelial growth factor in primary cultures of fetal rat calvaria cells

Lee Chuan C Yeh, John C. Lee

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Osteogenic protein-1 (OP-1 or BMP-7) stimulates new bone formation in vivo and induces cell proliferation and differentiation of osteoblasts in vitro. In the present study, we examined effects of OP-1 on the expression of vascular endothelial growth factor (VEGF) in primary cultures of fetal rat calvaria (FRC) cells. OP-1 increased the steady-state level of VEGF mRNA by about 3-fold in an OP-1 concentration- and time-dependent manner. The increase in VEGF mRNA level depended on transcription and was sensitive to cell replication. The VEGF mRNA stability was unaffected. The mRNA levels for both types of VEGF receptors, Flk-1 and Flt-1 were low but detectable in FRC cells by RT-PCR and were not changed by OP-1. Inhibition of VEGF synthesis and function by antisense oligonucleotide and by suramin, respectively arrested the OP-1-induced alkaline phosphatase activity and mineralized bone nodule formation. Together with published studies of VEGF on vascular endothelial cells which are usually found in close proximity to osteoblastic cells in vivo, these results suggest that VEGF participates in the OP-1- induced osteogenesis by taking part in bone cell differentiation and by promoting angiogenesis at the side of bone formation.

Original languageEnglish (US)
Pages (from-to)113-124
Number of pages12
JournalMolecular and Cellular Endocrinology
Volume153
Issue number1-2
DOIs
StatePublished - Jul 20 1999

Fingerprint

Bone Morphogenetic Protein 7
Skull
Gene expression
Vascular Endothelial Growth Factor A
Rats
Gene Expression
Osteogenesis
Bone
Messenger RNA
Cell Differentiation
Vascular Endothelial Growth Factor Receptor-1
Suramin
Vascular Endothelial Growth Factor Receptor
Antisense Oligonucleotides
Osteoblasts
Endothelial cells
RNA Stability
Cell proliferation
Transcription
Alkaline Phosphatase

Keywords

  • BMP
  • Osteoblast
  • Osteogenic protein-1
  • VEGF expression

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Osteogenic protein-1 increases gene expression of vascular endothelial growth factor in primary cultures of fetal rat calvaria cells. / Yeh, Lee Chuan C; Lee, John C.

In: Molecular and Cellular Endocrinology, Vol. 153, No. 1-2, 20.07.1999, p. 113-124.

Research output: Contribution to journalArticle

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