Osteogenic protein-1 enhances phenotypic expression in ROS 17/2.8 cells

A. M. Kitten, J. C. Lee, M. S. Olson

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Osteogenic protein-1 (OP-1) stimulates bone morphogenesis in vivo and modulates osteoblast growth and differentiation in vitro. Treatment of ROS 17/2.8 cells with OP-1 resulted in a time- and concentration-dependent inhibition of [3H]thymidine incorporation. In contrast, OP-1 treatment stimulated phenotypic differentiation in ROS 17/2.8 cells, as indicated by enhanced 1) alkaline phosphatase activity (4-fold); 2) alkaline phosphate mRNA (5-fold); 3) parathyroid hormone receptor mRNA (2-fold), and 4) parathyroid hormone-stimulated adenosine 3',5'-cyclic monophosphate accumulation (2-fold). OP-1-induced changes in cell growth and gene expression were sensitive to cycloheximide and actinomycin D. Measurement of [3H]thymidine incorporation and alkaline phosphatase activity in situ revealed heterogeneity in the cellular responses to OP-1. Proliferating cells exhibited less alkaline phosphatase activity than nonproliferating cells, whereas cells expressing high levels of alkaline phosphatase incorporated little [3H]thymidine. Our data delineating the responses of mature differentiated osteoblasts to OP-1 suggest that potentiation of osteoblast differentiated function is an important component of hone morphogenesis in vivo.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume269
Issue number5 32-5
StatePublished - 1995

Fingerprint

Bone Morphogenetic Protein 7
Alkaline Phosphatase
alkaline phosphatase
osteoblasts
Osteoblasts
thymidine
Thymidine
proteins
cells
Morphogenesis
Receptor, Parathyroid Hormone, Type 2
morphogenesis
Parathyroid Hormone Receptor Type 1
Messenger RNA
5'-Nucleotidase
actinomycin D
parathyroid hormone
Cell growth
cyclic AMP
Dactinomycin

Keywords

  • alkaline phosphatase
  • bone morphogenetic protein
  • deoxyribonucleic acid synthesis
  • gene expression
  • parathyroid hormone

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Osteogenic protein-1 enhances phenotypic expression in ROS 17/2.8 cells. / Kitten, A. M.; Lee, J. C.; Olson, M. S.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 269, No. 5 32-5, 1995.

Research output: Contribution to journalArticle

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