Osteogenic protein-1 enhances phenotypic expression in ROS 17/2.8 cells

A. M. Kitten, J. C. Lee, M. S. Olson

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Osteogenic protein-1 (OP-1) stimulates bone morphogenesis in vivo and modulates osteoblast growth and differentiation in vitro. Treatment of ROS 17/2.8 cells with OP-1 resulted in a time- and concentration-dependent inhibition of [3H]thymidine incorporation. In contrast, OP-1 treatment stimulated phenotypic differentiation in ROS 17/2.8 cells, as indicated by enhanced 1) alkaline phosphatase activity (4-fold); 2) alkaline phosphate mRNA (5-fold); 3) parathyroid hormone receptor mRNA (2-fold), and 4) parathyroid hormone-stimulated adenosine 3',5'-cyclic monophosphate accumulation (2-fold). OP-1-induced changes in cell growth and gene expression were sensitive to cycloheximide and actinomycin D. Measurement of [3H]thymidine incorporation and alkaline phosphatase activity in situ revealed heterogeneity in the cellular responses to OP-1. Proliferating cells exhibited less alkaline phosphatase activity than nonproliferating cells, whereas cells expressing high levels of alkaline phosphatase incorporated little [3H]thymidine. Our data delineating the responses of mature differentiated osteoblasts to OP-1 suggest that potentiation of osteoblast differentiated function is an important component of hone morphogenesis in vivo.

Original languageEnglish (US)
Pages (from-to)E918-E926
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume269
Issue number5 32-5
DOIs
StatePublished - Jan 1 1995

Keywords

  • alkaline phosphatase
  • bone morphogenetic protein
  • deoxyribonucleic acid synthesis
  • gene expression
  • parathyroid hormone

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

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