Osteogenic and osteoclastic cell interaction: Development of a co-culture system

Peter M. Loomer, Richard P. Ellen, Howard C. Tenenbaum

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The processes involved in the regulation of bone cell metabolism are complex, including those implicated in bone cell coupling. This study was undertaken to develop a model that would permit real-time interaction between osteoclastic cells and osteoblasts in vitro. Osteogenic bone marrow stromal cells were isolated from 18-day-old embryonic chickens, while osteoclastic cells were isolated from laying White Leghorn hens on calcium-deficient diets. Osteoclastic cells (5 x 105) were seeded onto mineral thin films and suspended above osteogenic cells (1 x 104) already plated on the bottoms of tissue culture plate wells. The data showed that after 4 days of incubation there was up to a fivefold (P < 0.05) reduction in all measured parameters of osteogenesis (mineralization, alkaline phosphatase activity and type I collagen production) in osteogenic cultures grown in the presence of osteoclastic cells. Similarly, osteoclastic cell-induced mineral resorption was reduced up to threefold (P < 0.05). Co-culture effects on cellular responses could be manipulated by known antiresorptive agents (e.g., pamidronate) altering either the source or the age of osteoclastic cells. The results indicate that the co-culture model may be useful in the study of bone cell interactions.

Original languageEnglish (US)
Pages (from-to)99-108
Number of pages10
JournalCell and Tissue Research
Volume294
Issue number1
DOIs
StatePublished - Oct 8 1998
Externally publishedYes

Keywords

  • Biomaterials
  • Bone formation
  • Chicken (culture)
  • Mineral resorption
  • Osteoblasts
  • Osteoclasts
  • Stromal cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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