Orthopedic and dental treatment commonly uses bone grafts to fill bone defects, facilitate bone repair, provide mechanical support, and stabilize a deformed area during bone regeneration. In combination with the bone grafting material, growth factors and proteins are used to enhance osteogenesis during bone regeneration. One of these bone-enhancing proteins is provided in enamel matrix derivative (EMD). Osteogain™ is a liquid formulation of EMD, which has been found to enhance periodontal regeneration in vivo and improve osteogenic differentiation of bone cells in vitro. It is not clear if combining Osteogain™ with bone grafting materials like demineralized bone matrix (DBM) will increase their osteoinductive potential. The liquid carrier system is promising because it provides increased surface loading for porous graft materials and allows more stable proteins surface coatings. The objective of this study was to determine whether Osteogain™ can increase the osteoinductive potential of DBM in vivo in an established mouse model of bone induction.