Osteoblast response to bioactive glasses in vitro correlates with inorganic phosphate content

S. Lossdörfer; Z. Schwartz; C. H. Lohmann; D. C. Greenspan; D. M. Ranly; B. D. Boyan

doi:10.1016/j.biomaterials.2003.09.094

Osteoblast response to bioactive glasses in vitro correlates with inorganic phosphate content

S. Lossdörfer, Z. Schwartz, C. H. Lohmann, D. C. Greenspan, D. M. Ranly, B. D. Boyan

Periodontics

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49 Scopus citations

Abstract

Inorganic phosphate (Pi) is a physiological regulator of osteoblasts and chondrocytes, suggesting that phosphate may contribute to the biological response of these cells to bioactive glasses like Bioglass® 45S5, which is composed of 45% SiO₂, 24.5% CaO, 24.5% Na₂O, and 6% P ₂O₅. We investigated the effect of varying the Pi content of bioactive glass disks (0%, 3%, 6% and 12% P₂O₅) using human osteoblast-like MG63 cells as the model. Cell number on 6% Pi disks was comparable to cultures on tissue culture plastic, but was reduced at higher and lower Pi concentrations. Alkaline phosphatase specific activity of isolated cells and cell layer lysates, as well as PGE₂, TGF-β ₁ and NO levels in conditioned media, were elevated in cultures grown on bioactive glass and varied with the Pi content. The greatest effects were observed in cultures grown on disks with the lowest Pi concentrations. Thus, growth on the bioactive glasses enhances cell function in comparison with tissue culture plastic and lower Pi content favors osteoblast differentiation.

Original language	English (US)
Pages (from-to)	2547-2555
Number of pages	9
Journal	Biomaterials
Volume	25
Issue number	13
DOIs	https://doi.org/10.1016/j.biomaterials.2003.09.094
State	Published - Jun 2004

Keywords

Bioactive glass
Bioglass®
Differentiation
In vitro
Inorganic phosphate
Local factor production
MG63 osteoblast-like cells
Proliferation

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

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title = "Osteoblast response to bioactive glasses in vitro correlates with inorganic phosphate content",

abstract = "Inorganic phosphate (Pi) is a physiological regulator of osteoblasts and chondrocytes, suggesting that phosphate may contribute to the biological response of these cells to bioactive glasses like Bioglass{\textregistered} 45S5, which is composed of 45% SiO2, 24.5% CaO, 24.5% Na2O, and 6% P 2O5. We investigated the effect of varying the Pi content of bioactive glass disks (0%, 3%, 6% and 12% P2O5) using human osteoblast-like MG63 cells as the model. Cell number on 6% Pi disks was comparable to cultures on tissue culture plastic, but was reduced at higher and lower Pi concentrations. Alkaline phosphatase specific activity of isolated cells and cell layer lysates, as well as PGE2, TGF-β 1 and NO levels in conditioned media, were elevated in cultures grown on bioactive glass and varied with the Pi content. The greatest effects were observed in cultures grown on disks with the lowest Pi concentrations. Thus, growth on the bioactive glasses enhances cell function in comparison with tissue culture plastic and lower Pi content favors osteoblast differentiation.",

keywords = "Bioactive glass, Bioglass{\textregistered}, Differentiation, In vitro, Inorganic phosphate, Local factor production, MG63 osteoblast-like cells, Proliferation",

author = "S. Lossd{\"o}rfer and Z. Schwartz and Lohmann, {C. H.} and Greenspan, {D. C.} and Ranly, {D. M.} and Boyan, {B. D.}",

note = "Funding Information: Dr. S. Lossd{\"o}rfer was supported by a grant from the Postdoctoral Fellowship Program of the German Academic Exchange Service (DAAD). The authors thank Wanda Whitfield for her help in preparing the manuscript. US Biomaterials, Inc. (Alachua, FL) fabricated the disks and contributed them to the study. This research was supported by the Center for the Enhancement of the Biology/Biomaterials Interface at the University of Texas Health Science Center at San Antonio, The Georgia Research Alliance, and the Georgia Tech/Emory Center for the Engineering of Living Tissues, Atlanta, Georgia. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2004",

month = jun,

doi = "10.1016/j.biomaterials.2003.09.094",

language = "English (US)",

volume = "25",

pages = "2547--2555",

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T1 - Osteoblast response to bioactive glasses in vitro correlates with inorganic phosphate content

AU - Lossdörfer, S.

AU - Schwartz, Z.

AU - Lohmann, C. H.

AU - Greenspan, D. C.

AU - Ranly, D. M.

AU - Boyan, B. D.

N1 - Funding Information: Dr. S. Lossdörfer was supported by a grant from the Postdoctoral Fellowship Program of the German Academic Exchange Service (DAAD). The authors thank Wanda Whitfield for her help in preparing the manuscript. US Biomaterials, Inc. (Alachua, FL) fabricated the disks and contributed them to the study. This research was supported by the Center for the Enhancement of the Biology/Biomaterials Interface at the University of Texas Health Science Center at San Antonio, The Georgia Research Alliance, and the Georgia Tech/Emory Center for the Engineering of Living Tissues, Atlanta, Georgia. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2004/6

Y1 - 2004/6

N2 - Inorganic phosphate (Pi) is a physiological regulator of osteoblasts and chondrocytes, suggesting that phosphate may contribute to the biological response of these cells to bioactive glasses like Bioglass® 45S5, which is composed of 45% SiO2, 24.5% CaO, 24.5% Na2O, and 6% P 2O5. We investigated the effect of varying the Pi content of bioactive glass disks (0%, 3%, 6% and 12% P2O5) using human osteoblast-like MG63 cells as the model. Cell number on 6% Pi disks was comparable to cultures on tissue culture plastic, but was reduced at higher and lower Pi concentrations. Alkaline phosphatase specific activity of isolated cells and cell layer lysates, as well as PGE2, TGF-β 1 and NO levels in conditioned media, were elevated in cultures grown on bioactive glass and varied with the Pi content. The greatest effects were observed in cultures grown on disks with the lowest Pi concentrations. Thus, growth on the bioactive glasses enhances cell function in comparison with tissue culture plastic and lower Pi content favors osteoblast differentiation.

AB - Inorganic phosphate (Pi) is a physiological regulator of osteoblasts and chondrocytes, suggesting that phosphate may contribute to the biological response of these cells to bioactive glasses like Bioglass® 45S5, which is composed of 45% SiO2, 24.5% CaO, 24.5% Na2O, and 6% P 2O5. We investigated the effect of varying the Pi content of bioactive glass disks (0%, 3%, 6% and 12% P2O5) using human osteoblast-like MG63 cells as the model. Cell number on 6% Pi disks was comparable to cultures on tissue culture plastic, but was reduced at higher and lower Pi concentrations. Alkaline phosphatase specific activity of isolated cells and cell layer lysates, as well as PGE2, TGF-β 1 and NO levels in conditioned media, were elevated in cultures grown on bioactive glass and varied with the Pi content. The greatest effects were observed in cultures grown on disks with the lowest Pi concentrations. Thus, growth on the bioactive glasses enhances cell function in comparison with tissue culture plastic and lower Pi content favors osteoblast differentiation.

KW - Bioactive glass

KW - Bioglass®

KW - Differentiation

KW - In vitro

KW - Inorganic phosphate

KW - Local factor production

KW - MG63 osteoblast-like cells

KW - Proliferation

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JO - Biomaterials

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Osteoblast response to bioactive glasses in vitro correlates with inorganic phosphate content

Abstract

Keywords

ASJC Scopus subject areas

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