Organo-selenium-containing dental sealant inhibits bacterial biofilm

P. Tran, A. Hamood, T. Mosley, T. Gray, C. Jarvis, D. Webster, B. Amaechi, T. Enos, T. Reid

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Oral bacteria, including Streptococcus mutans and Streptococcus salivarius, contribute to tooth decay and plaque formation; therefore, it is essential to develop strategies to prevent dental caries and plaque formation. We recently showed that organo-selenium compounds covalently attached to different biomaterials inhibited bacterial biofilms. Our current study investigates the efficacy of an organo-selenium dental sealant (SeLECT-Defense™ sealant) in inhibiting S. mutans and S. salivarius biofilm formation in vitro. The organo-selenium was synthesized and covalently attached to dental sealant material via standard polymer chemistry. By colony-forming unit (CFU) assay and confocal microscopy, SeLECT-Defense™ sealant was found to completely inhibit the development of S. mutans and S. salivarius biofilms. To assess the durability of the anti-biofilm effect, we soaked the SeLECT-Defense™ sealant in PBS for 2 mos at 37 °C and found that the biofilm-inhibitory effect was not diminished after soaking. To determine if organo-selenium inhibits bacterial growth under the sealant, we placed SeLECT-Defense sealant over a lawn of S. mutans. In contrast to a control sealant, SeLECT- Defense™ sealant completely inhibited the growth of S. mutans. These results suggest that the inhibitory effect of SeLECT-Defense™ sealant against S. mutans and S. salivarius biofilms is very effective and durable.

Original languageEnglish (US)
Pages (from-to)461-466
Number of pages6
JournalJournal of dental research
Issue number5
StatePublished - May 2013


  • SeLECT-Defense™
  • dental caries
  • dental plaque
  • dental sealant
  • organo-selenium
  • selenium-compounds

ASJC Scopus subject areas

  • Dentistry(all)


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