Organic acid secretory pathway and urinary excretion of prostaglandin E in the dog

S. G. Rosenblatt, R. V. Patak, M. D. Lifschitz

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25 Scopus citations


Urinary prostaglandin E (PGE) has been utilized as an index of renal PGE production. Recent studies, however, have suggested that the organic acid secretory pathway is a major determinant of endogenous U(PGE)V (urinary excretion of PGE). The following experiments were designed to quantitatively test this latter view. In clearance studies the administration of para-aminohippurate (PAH) or probenecid (25 mg/kg) failed to alter endogenous U(PGE)V although PAH clearance fell with probenecid. Comparison of the excretion patterns of exogenously administered [3H]PGE and [14C]inulin after intra-arterial injection into the dog renal artery (Chinard technique) demonstrated both glomerular filtration and secretion of [3H]PGE. BLockade of the organic acid pathway by probenecid (25 mg/kg) abolished [3H]PGE secretion. Indomethacin (1 mg/kg) did not alter the secretion pattern of [3H]PGE, but decreased endogenous U(PGE)V by over 80%. Because this low dose of indomethacin did not decrease [3H]PGE secretion, it probably decreased endogenous U(PGE)V by inhibiting synthesis. Thus, these tracer studies do indeed confirm the suggestion that PGE may be excreted by the organic acid pathway. However, the failure of total endogenous U(PGE)V to fall after blockade of the organic acid pathway would suggest that the component of U(PGE)V due to this mechanism is quantitatively insignificant.

Original languageEnglish (US)
Pages (from-to)F473-F479
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Issue number5
StatePublished - 1978

ASJC Scopus subject areas

  • Physiology


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