Oral vaccination of racoons (Procyon lotor) with baculovirus-expressed rabies virus glycoprotein

Zhen Fang Fu; Charles E. Rupprecht; Bernhard Dietzschold; Potham Saikumar; Hong Shun Niu; Ildiko Babka; William H. Wunner; Hilary Koprowski

doi:10.1016/0264-410X(93)90379-C

Oral vaccination of racoons (Procyon lotor) with baculovirus-expressed rabies virus glycoprotein

Zhen Fang Fu, Charles E. Rupprecht, Bernhard Dietzschold, Potham Saikumar, Hong Shun Niu, Ildiko Babka, William H. Wunner, Hilary Koprowski

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Successful field oral vaccination and protection against viral diseases have so far been achieved only with live-attenuated or live-recombinant virus vaccines. In this communication, we present data that demonstrate that a glycoprotein derived from recombinant baculovirus-infected insect cells is efficacious as an oral vaccine. The glycoprotein (G) of rabies virus (Evelyn Rokitnicki Abelseth strain) was abundantly expressed in a baculovirus expression system and oral vaccination of racoons with the baculovirus-expressed G protein resulted in the production of rabies virus-neutralizing antibodies and protection against a lethal challenge with a street rabies virus. The potential for using the baculovirus-expressed G protein for oral immunization of wildlife is discussed.

Original language	English (US)
Pages (from-to)	925-928
Number of pages	4
Journal	Vaccine
Volume	11
Issue number	9
DOIs	https://doi.org/10.1016/0264-410X(93)90379-C
State	Published - 1993
Externally published	Yes

Keywords

Rabies
baculovirus
glycoprotein
oral vaccination
wildlife

ASJC Scopus subject areas

Molecular Medicine
Immunology and Microbiology(all)
veterinary(all)
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/0264-410X(93)90379-C

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title = "Oral vaccination of racoons (Procyon lotor) with baculovirus-expressed rabies virus glycoprotein",

abstract = "Successful field oral vaccination and protection against viral diseases have so far been achieved only with live-attenuated or live-recombinant virus vaccines. In this communication, we present data that demonstrate that a glycoprotein derived from recombinant baculovirus-infected insect cells is efficacious as an oral vaccine. The glycoprotein (G) of rabies virus (Evelyn Rokitnicki Abelseth strain) was abundantly expressed in a baculovirus expression system and oral vaccination of racoons with the baculovirus-expressed G protein resulted in the production of rabies virus-neutralizing antibodies and protection against a lethal challenge with a street rabies virus. The potential for using the baculovirus-expressed G protein for oral immunization of wildlife is discussed.",

keywords = "Rabies, baculovirus, glycoprotein, oral vaccination, wildlife",

author = "Fu, {Zhen Fang} and Rupprecht, {Charles E.} and Bernhard Dietzschold and Potham Saikumar and Niu, {Hong Shun} and Ildiko Babka and Wunner, {William H.} and Hilary Koprowski",

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T1 - Oral vaccination of racoons (Procyon lotor) with baculovirus-expressed rabies virus glycoprotein

AU - Fu, Zhen Fang

AU - Rupprecht, Charles E.

AU - Dietzschold, Bernhard

AU - Saikumar, Potham

AU - Niu, Hong Shun

AU - Babka, Ildiko

AU - Wunner, William H.

AU - Koprowski, Hilary

PY - 1993

Y1 - 1993

N2 - Successful field oral vaccination and protection against viral diseases have so far been achieved only with live-attenuated or live-recombinant virus vaccines. In this communication, we present data that demonstrate that a glycoprotein derived from recombinant baculovirus-infected insect cells is efficacious as an oral vaccine. The glycoprotein (G) of rabies virus (Evelyn Rokitnicki Abelseth strain) was abundantly expressed in a baculovirus expression system and oral vaccination of racoons with the baculovirus-expressed G protein resulted in the production of rabies virus-neutralizing antibodies and protection against a lethal challenge with a street rabies virus. The potential for using the baculovirus-expressed G protein for oral immunization of wildlife is discussed.

AB - Successful field oral vaccination and protection against viral diseases have so far been achieved only with live-attenuated or live-recombinant virus vaccines. In this communication, we present data that demonstrate that a glycoprotein derived from recombinant baculovirus-infected insect cells is efficacious as an oral vaccine. The glycoprotein (G) of rabies virus (Evelyn Rokitnicki Abelseth strain) was abundantly expressed in a baculovirus expression system and oral vaccination of racoons with the baculovirus-expressed G protein resulted in the production of rabies virus-neutralizing antibodies and protection against a lethal challenge with a street rabies virus. The potential for using the baculovirus-expressed G protein for oral immunization of wildlife is discussed.

KW - Rabies

KW - baculovirus

KW - glycoprotein

KW - oral vaccination

KW - wildlife

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