Abstract
Successful field oral vaccination and protection against viral diseases have so far been achieved only with live-attenuated or live-recombinant virus vaccines. In this communication, we present data that demonstrate that a glycoprotein derived from recombinant baculovirus-infected insect cells is efficacious as an oral vaccine. The glycoprotein (G) of rabies virus (Evelyn Rokitnicki Abelseth strain) was abundantly expressed in a baculovirus expression system and oral vaccination of racoons with the baculovirus-expressed G protein resulted in the production of rabies virus-neutralizing antibodies and protection against a lethal challenge with a street rabies virus. The potential for using the baculovirus-expressed G protein for oral immunization of wildlife is discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 925-928 |
| Number of pages | 4 |
| Journal | Vaccine |
| Volume | 11 |
| Issue number | 9 |
| DOIs | |
| State | Published - 1993 |
| Externally published | Yes |
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Keywords
- baculovirus
- glycoprotein
- oral vaccination
- Rabies
- wildlife
ASJC Scopus subject areas
- Immunology
- Microbiology
- Virology
- Infectious Diseases
- Public Health, Environmental and Occupational Health
- veterinary(all)
Cite this
Oral vaccination of racoons (Procyon lotor) with baculovirus-expressed rabies virus glycoprotein. / Fu, Zhen Fang; Rupprecht, Charles E.; Dietzschold, Bernhard; Saikumar, Pothana; Niu, Hong Shun; Babka, Ildiko; Wunner, William H.; Koprowski, Hilary.
In: Vaccine, Vol. 11, No. 9, 1993, p. 925-928.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oral vaccination of racoons (Procyon lotor) with baculovirus-expressed rabies virus glycoprotein
AU - Fu, Zhen Fang
AU - Rupprecht, Charles E.
AU - Dietzschold, Bernhard
AU - Saikumar, Pothana
AU - Niu, Hong Shun
AU - Babka, Ildiko
AU - Wunner, William H.
AU - Koprowski, Hilary
PY - 1993
Y1 - 1993
N2 - Successful field oral vaccination and protection against viral diseases have so far been achieved only with live-attenuated or live-recombinant virus vaccines. In this communication, we present data that demonstrate that a glycoprotein derived from recombinant baculovirus-infected insect cells is efficacious as an oral vaccine. The glycoprotein (G) of rabies virus (Evelyn Rokitnicki Abelseth strain) was abundantly expressed in a baculovirus expression system and oral vaccination of racoons with the baculovirus-expressed G protein resulted in the production of rabies virus-neutralizing antibodies and protection against a lethal challenge with a street rabies virus. The potential for using the baculovirus-expressed G protein for oral immunization of wildlife is discussed.
AB - Successful field oral vaccination and protection against viral diseases have so far been achieved only with live-attenuated or live-recombinant virus vaccines. In this communication, we present data that demonstrate that a glycoprotein derived from recombinant baculovirus-infected insect cells is efficacious as an oral vaccine. The glycoprotein (G) of rabies virus (Evelyn Rokitnicki Abelseth strain) was abundantly expressed in a baculovirus expression system and oral vaccination of racoons with the baculovirus-expressed G protein resulted in the production of rabies virus-neutralizing antibodies and protection against a lethal challenge with a street rabies virus. The potential for using the baculovirus-expressed G protein for oral immunization of wildlife is discussed.
KW - baculovirus
KW - glycoprotein
KW - oral vaccination
KW - Rabies
KW - wildlife
UR - http://www.scopus.com/inward/record.url?scp=0027170656&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027170656&partnerID=8YFLogxK
U2 - 10.1016/0264-410X(93)90379-C
DO - 10.1016/0264-410X(93)90379-C
M3 - Article
C2 - 8212838
AN - SCOPUS:0027170656
VL - 11
SP - 925
EP - 928
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 9
ER -