Oral vaccination of racoons (Procyon lotor) with baculovirus-expressed rabies virus glycoprotein

Zhen Fang Fu, Charles E. Rupprecht, Bernhard Dietzschold, Pothana Saikumar, Hong Shun Niu, Ildiko Babka, William H. Wunner, Hilary Koprowski

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Successful field oral vaccination and protection against viral diseases have so far been achieved only with live-attenuated or live-recombinant virus vaccines. In this communication, we present data that demonstrate that a glycoprotein derived from recombinant baculovirus-infected insect cells is efficacious as an oral vaccine. The glycoprotein (G) of rabies virus (Evelyn Rokitnicki Abelseth strain) was abundantly expressed in a baculovirus expression system and oral vaccination of racoons with the baculovirus-expressed G protein resulted in the production of rabies virus-neutralizing antibodies and protection against a lethal challenge with a street rabies virus. The potential for using the baculovirus-expressed G protein for oral immunization of wildlife is discussed.

Original languageEnglish (US)
Pages (from-to)925-928
Number of pages4
JournalVaccine
Volume11
Issue number9
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Raccoons
oral vaccination
Rabies virus
Procyon lotor
Baculoviridae
glycoproteins
Glycoproteins
Vaccination
G-proteins
GTP-Binding Proteins
vaccines
Synthetic Vaccines
Virus Diseases
Neutralizing Antibodies
neutralizing antibodies
Insects
mouth
Immunization
wildlife
Vaccines

Keywords

  • baculovirus
  • glycoprotein
  • oral vaccination
  • Rabies
  • wildlife

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Fu, Z. F., Rupprecht, C. E., Dietzschold, B., Saikumar, P., Niu, H. S., Babka, I., ... Koprowski, H. (1993). Oral vaccination of racoons (Procyon lotor) with baculovirus-expressed rabies virus glycoprotein. Vaccine, 11(9), 925-928. https://doi.org/10.1016/0264-410X(93)90379-C

Oral vaccination of racoons (Procyon lotor) with baculovirus-expressed rabies virus glycoprotein. / Fu, Zhen Fang; Rupprecht, Charles E.; Dietzschold, Bernhard; Saikumar, Pothana; Niu, Hong Shun; Babka, Ildiko; Wunner, William H.; Koprowski, Hilary.

In: Vaccine, Vol. 11, No. 9, 1993, p. 925-928.

Research output: Contribution to journalArticle

Fu, ZF, Rupprecht, CE, Dietzschold, B, Saikumar, P, Niu, HS, Babka, I, Wunner, WH & Koprowski, H 1993, 'Oral vaccination of racoons (Procyon lotor) with baculovirus-expressed rabies virus glycoprotein', Vaccine, vol. 11, no. 9, pp. 925-928. https://doi.org/10.1016/0264-410X(93)90379-C
Fu, Zhen Fang ; Rupprecht, Charles E. ; Dietzschold, Bernhard ; Saikumar, Pothana ; Niu, Hong Shun ; Babka, Ildiko ; Wunner, William H. ; Koprowski, Hilary. / Oral vaccination of racoons (Procyon lotor) with baculovirus-expressed rabies virus glycoprotein. In: Vaccine. 1993 ; Vol. 11, No. 9. pp. 925-928.
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