Optoacoustic sensor for nanoparticle linked immunosorbent assay (NanoLISA)

André Conjusteau, Anton Liopo, Dmitri Tsyboulski, Sergey A. Ermilov, William R. Elliott, Norman Barsalou, Saher M. Maswadi, Randolph D. Glickman, Alexander A. Oraevsky

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

We developed an optoacoustic biosensor intended for the detection of bloodborne microorganisms using immunoaffinity reactions of antibody-coupled gold nanorods as contrast agents specifically targeted to the antigen of interest. Optoacoustic responses generated by the samples are detected using a wide band ultrasonic transducer. The sensitivity of the technique has been assessed by determining minimally detectable optical density which corresponds to the minimum detectable concentration of the target viral surface antigens. Both ionic solutions and gold nanorods served as the contrast agent generating the optoacoustic response. The sensitivity of Nano-LISA is at least OD=10 -6 which allows reliable detection of 1 pg/ml (depending on the commercial antibodies that are used). Adequate detection sensitivity, as well as lack of non-specific cross-reaction between antigens favors NanoLISA as a viable technology for biosensor development.

Original languageEnglish (US)
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2011
DOIs
StatePublished - May 5 2011
EventPhotons Plus Ultrasound: Imaging and Sensing 2011 - San Francisco, CA, United States
Duration: Jan 23 2011Jan 25 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7899
ISSN (Print)1605-7422

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing 2011
CountryUnited States
CitySan Francisco, CA
Period1/23/111/25/11

Keywords

  • Contrast agents
  • Gold nanoparticle conjugates
  • NanoLISA
  • Optoacoustic detection
  • Specific binding

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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