Optimizing transformation frequency of cryptococcus neoformans and cryptococcus gattii using agrobacterium tumefaciens

Jianmin Fu, Nohelli E. Brockman, Brian L. Wickes

Research output: Contribution to journalReview articlepeer-review

Abstract

The transformation of Cryptococcus spp. by Agrobacterium tumefaciens has proven to be a useful genetic tool. A number of factors affect transformation frequency. These factors include acetosyringone concentration, bacterial cell to yeast cell ratio, cell wall damage, and agar concentration. Agar concentration was found to have a significant effect on the transformant number as transformants increased with agar concentration across all four serotypes. When infection time points were tested, higher agar concentrations were found to result in an earlier transfer of the Ti-plasmid to the yeast cell, with the earliest transformant appearing two h after A. tumefaciens contact with yeast cells. These results demonstrate that A. tumefaciens transformation efficiency can be affected by a variety of factors and continued investigation of these factors can lead to improvements in specific A. tumefaciens/fungus transformation systems.

Original languageEnglish (US)
Article number520
JournalJournal of Fungi
Volume7
Issue number7
DOIs
StatePublished - Jul 2021

Keywords

  • Bacterial
  • Ti-plasmid
  • Transformation

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Microbiology (medical)

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