Optimized cell transplantation using adult rag2 mutant zebrafsh

Qin Tang, Nouran S. Abdelfattah, Jessica S. Blackburn, John C. Moore, Sarah A. Martinez, Finola E. Moore, Riadh Lobbardi, Inês M. Tenente, Myron S. Ignatius, Jason N. Berman, Robert S. Liwski, Yariv Houvras, David M. Langenau

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

Cell transplantation into adult zebrafsh has lagged behind mouse models owing to the lack of immunocompromised strains. here we have created rag2E450fs mutant zebrafsh that have reduced numbers of functional t and b cells but are viable and fecund. mutant fsh engraft muscle, blood stem cells and various cancers. rag2E450fs mutant zebrafsh are the frst immunocompromised zebrafsh model that permits robust, long-term engraftment of multiple tissues and cancer.

Original languageEnglish (US)
Pages (from-to)821-824
Number of pages4
JournalNature Methods
Volume11
Issue number8
DOIs
StatePublished - Aug 2014
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Tang, Q., Abdelfattah, N. S., Blackburn, J. S., Moore, J. C., Martinez, S. A., Moore, F. E., Lobbardi, R., Tenente, I. M., Ignatius, M. S., Berman, J. N., Liwski, R. S., Houvras, Y., & Langenau, D. M. (2014). Optimized cell transplantation using adult rag2 mutant zebrafsh. Nature Methods, 11(8), 821-824. https://doi.org/10.1038/nmeth.3031