Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach

Steven L. Farnsworth, Zhifang Qiu, Anuja Mishra, Peter J Hornsby

Research output: Contribution to journalArticle

Abstract

The directed differentiation of pluripotent stem cells to a desired lineage often involves complex and lengthy protocols. In order to study the requirements for differentiation in a systematic way, we present here methodology for an iterative approach using combinations of small molecules and biological factors. The factors are used in a cyclical process in which the best combination of factors and concentrations is selected in one round of testing, followed by a modification of the combination and subsequent rounds. While this may produce the desired differentiation in the cell population under study, it is also possible that other strategies may be needed to optimize the differentiation process. These strategies are described in this chapter.

Original languageEnglish (US)
Pages (from-to)187-197
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume1919
DOIs
StatePublished - Jan 1 2019

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Keywords

  • Algorithms
  • Differentiation
  • Gene expression
  • Nonhuman primates
  • Pluripotent stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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