Ontogeny of angiotensin II type 1 receptor and cytochrome P450(c11) in the sheep adrenal gland

Catherine L. Coulter, Dean A. Myers, Peter W. Nathanielsz, Ian M. Bird

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

In the present study we investigated the ontogeny of the expression of the type 1 angiotensin receptor (AT1R mRNA) and the zonal localization of AT1R immunoreactivity (AT1R-ir) and cytochrome P450(c11) (CYP11B-ir) in the sheep adrenal gland. In the adult sheep and in the fetus from as early as 90 days gestation, intense AT1R-ir was observed predominantly in the zona glomerulosa and to a lesser extent in the zona fasciculata, and it was not detectable in the adrenal medulla. AT1R mRNA decreased 4-fold between 105 days and 120 days, whereas AT1R mRNA levels remained relatively constant between 120 days and the newborn period. In contrast, both in the adult sheep and in the fetal sheep from as early as 90 days gestation, intense CYP11B-ir was consistently detected throughout the adrenal cortex and in steroidogenic cells that surround the central adrenal vein. In conclusion, we speculate that the presence of AT1R in the zona fasciculata, and the higher levels of expression of AT1R at around 100 days gestation, may suggest that suppression of CYP17 is mediated via AT1R at this time. The abundant expression of AT1R-ir and CYP11B-ir in the zona glomerulosa of the fetal sheep adrenal gland would also suggest that lack of angiotensin II stimulation of aldosterone secretion is not due to an absence of AT1R or CYP11B in the zona glomerulosa.

Original languageEnglish (US)
Pages (from-to)714-719
Number of pages6
JournalBiology of reproduction
Volume62
Issue number3
DOIs
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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