Omega-hydroxylation of prostaglandin E1 in the isolated perfused lungs of pregnant rabbits

Marie T. Leithauser, David L. Roerig, Sharon M. Winquist, Arnold Gee, Richard T. Okita, Bettie Su Siler Masters

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Abstract

Cytochrome P450PGω is induced in the rabbit lung in a gestational age-dependent manner and hydroxylates certain eicosanoids at their terminal, or omega (ω), carbon. This enzyme has beenisolated from microsomal fractions and its activity has been characterized (Williams, D.E., et al., J. Biol. Chem. 259; 14600-14608, 1984). The experiments presented here examine the ω-hydroxylation activity of the intact lung during presentation of an eicosanoid substrate, prostaglandin E1 (PGE1), to the lung vasculature. Isolated, perfused lungs from three pregnant and four nonpregnatn rabbits were injected with [3H]-PGE1. One-second fractions were collected from the perfusion effluent and were analyzed for metabolism of PGE1. Lungs isolated from pregnant rabbits metabolized PGE1 mainly to two polar derivatives, 20-hydroxy-PGE1 and 13,14-dihydro-15-keto-20-hydroxy-PGE1, whereas lungs from nonpregnant rabbits yielded mainly a relatively nonpolar metabolite, 13,14-dihydro-15-keto-PGE1. These metabolites were identified by coelution with standards that were generated enzymatically in vitro and whose structures were confirmed by gas chromatography/mass spectrometry (GC/MS).

Original languageEnglish (US)
Pages (from-to)819-833
Number of pages15
JournalProstaglandins
Volume36
Issue number6
DOIs
StatePublished - Dec 1988

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ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Leithauser, M. T., Roerig, D. L., Winquist, S. M., Gee, A., Okita, R. T., & Su Siler Masters, B. (1988). Omega-hydroxylation of prostaglandin E1 in the isolated perfused lungs of pregnant rabbits. Prostaglandins, 36(6), 819-833. https://doi.org/10.1016/0090-6980(88)90059-7