Omega-3 fatty acid regulates inflammatory cytokine/mediator messenger RNA expression in Porphyromonas gingivalis-induced experimental periodontal disease

L. Kesavalu, V. Bakthavatchalu, M. M. Rahman, J. Su, B. Raghu, D. Dawson, G. Fernandes, J. L. Ebersole

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Introduction: Porphyromonas gingivalis is strongly implicated in the etiology of adult periodontitis by inducing inflammatory cytokines, resulting in gingival and periodontal tissue inflammation and alveolar bone resorption. This study tested the hypothesis that supplementing the diet with omega-3 fatty acid (ω-3 FA; i.e. fish oil) would exert anti-inflammatory effects in the gingival tissues of P. gingivalis-infected rats. Methods: Rats were fed either fish oil or corn oil diets ad libitum for 22 weeks and infected with P. gingivalis strain 381 or strain A7A1-28. After sacrifice, rat gingival tissues were excised and the RNA was isolated and analyzed for proinflammatory mediators [interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), IL-6], T helper type 1 and type 2 cytokines [interferon-γ (IFN-γ), IL-4, IL-10), antioxidant enzymes [catalase (CAT), superoxide dismutase (SOD)], and genes critical for eicosanoid mediator production [cyclo-oxygenase-2 (COX-2), 5-lipoxygenase (5-LO)] by reverse transcription-polymerase chain reaction using rat-specific primers. Results: Rats on the ω-3 FA diet exhibited decreased proinflammatory cytokine gene expression (IL-1β, TNF-α) and enhanced IFN-γ, CAT and SOD messenger RNA expression compared to rats fed a corn oil diet, supporting a diet-induced modulation of host inflammatory reactions. Analyses of alveolar bone resorption in the rats related to gene expression profiles demonstrated significant positive correlations with IL-1β, IL-6 and COX-2 and negative correlations with CAT and SOD. Conclusion: These findings suggest that diets enriched for ω-3 FA modulate the local gingival inflammatory milieu of the host following oral P. gingivalis infection, which impacts on alveolar bone resorption in rats.

Original languageEnglish (US)
Pages (from-to)232-239
Number of pages8
JournalOral Microbiology and Immunology
Volume22
Issue number4
DOIs
StatePublished - Aug 2007

Fingerprint

Porphyromonas gingivalis
Omega-3 Fatty Acids
Periodontal Diseases
Cytokines
Messenger RNA
Diet
Alveolar Bone Loss
Bone Resorption
Interleukin-1
Catalase
Superoxide Dismutase
Corn Oil
Fish Oils
Prostaglandin-Endoperoxide Synthases
Interleukin-6
Tumor Necrosis Factor-alpha
Arachidonate 5-Lipoxygenase
Chronic Periodontitis
Eicosanoids
Transcriptome

Keywords

  • Gene expression
  • Inflammatory cytokine
  • Omega-3 fatty acid
  • Periodontal disease
  • Porphyromonas gingivalis
  • Rat model
  • Reverse transcription-polymerase chain reaction

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)
  • Dentistry(all)

Cite this

Omega-3 fatty acid regulates inflammatory cytokine/mediator messenger RNA expression in Porphyromonas gingivalis-induced experimental periodontal disease. / Kesavalu, L.; Bakthavatchalu, V.; Rahman, M. M.; Su, J.; Raghu, B.; Dawson, D.; Fernandes, G.; Ebersole, J. L.

In: Oral Microbiology and Immunology, Vol. 22, No. 4, 08.2007, p. 232-239.

Research output: Contribution to journalArticle

Kesavalu, L. ; Bakthavatchalu, V. ; Rahman, M. M. ; Su, J. ; Raghu, B. ; Dawson, D. ; Fernandes, G. ; Ebersole, J. L. / Omega-3 fatty acid regulates inflammatory cytokine/mediator messenger RNA expression in Porphyromonas gingivalis-induced experimental periodontal disease. In: Oral Microbiology and Immunology. 2007 ; Vol. 22, No. 4. pp. 232-239.
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abstract = "Introduction: Porphyromonas gingivalis is strongly implicated in the etiology of adult periodontitis by inducing inflammatory cytokines, resulting in gingival and periodontal tissue inflammation and alveolar bone resorption. This study tested the hypothesis that supplementing the diet with omega-3 fatty acid (ω-3 FA; i.e. fish oil) would exert anti-inflammatory effects in the gingival tissues of P. gingivalis-infected rats. Methods: Rats were fed either fish oil or corn oil diets ad libitum for 22 weeks and infected with P. gingivalis strain 381 or strain A7A1-28. After sacrifice, rat gingival tissues were excised and the RNA was isolated and analyzed for proinflammatory mediators [interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), IL-6], T helper type 1 and type 2 cytokines [interferon-γ (IFN-γ), IL-4, IL-10), antioxidant enzymes [catalase (CAT), superoxide dismutase (SOD)], and genes critical for eicosanoid mediator production [cyclo-oxygenase-2 (COX-2), 5-lipoxygenase (5-LO)] by reverse transcription-polymerase chain reaction using rat-specific primers. Results: Rats on the ω-3 FA diet exhibited decreased proinflammatory cytokine gene expression (IL-1β, TNF-α) and enhanced IFN-γ, CAT and SOD messenger RNA expression compared to rats fed a corn oil diet, supporting a diet-induced modulation of host inflammatory reactions. Analyses of alveolar bone resorption in the rats related to gene expression profiles demonstrated significant positive correlations with IL-1β, IL-6 and COX-2 and negative correlations with CAT and SOD. Conclusion: These findings suggest that diets enriched for ω-3 FA modulate the local gingival inflammatory milieu of the host following oral P. gingivalis infection, which impacts on alveolar bone resorption in rats.",
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T1 - Omega-3 fatty acid regulates inflammatory cytokine/mediator messenger RNA expression in Porphyromonas gingivalis-induced experimental periodontal disease

AU - Kesavalu, L.

AU - Bakthavatchalu, V.

AU - Rahman, M. M.

AU - Su, J.

AU - Raghu, B.

AU - Dawson, D.

AU - Fernandes, G.

AU - Ebersole, J. L.

PY - 2007/8

Y1 - 2007/8

N2 - Introduction: Porphyromonas gingivalis is strongly implicated in the etiology of adult periodontitis by inducing inflammatory cytokines, resulting in gingival and periodontal tissue inflammation and alveolar bone resorption. This study tested the hypothesis that supplementing the diet with omega-3 fatty acid (ω-3 FA; i.e. fish oil) would exert anti-inflammatory effects in the gingival tissues of P. gingivalis-infected rats. Methods: Rats were fed either fish oil or corn oil diets ad libitum for 22 weeks and infected with P. gingivalis strain 381 or strain A7A1-28. After sacrifice, rat gingival tissues were excised and the RNA was isolated and analyzed for proinflammatory mediators [interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), IL-6], T helper type 1 and type 2 cytokines [interferon-γ (IFN-γ), IL-4, IL-10), antioxidant enzymes [catalase (CAT), superoxide dismutase (SOD)], and genes critical for eicosanoid mediator production [cyclo-oxygenase-2 (COX-2), 5-lipoxygenase (5-LO)] by reverse transcription-polymerase chain reaction using rat-specific primers. Results: Rats on the ω-3 FA diet exhibited decreased proinflammatory cytokine gene expression (IL-1β, TNF-α) and enhanced IFN-γ, CAT and SOD messenger RNA expression compared to rats fed a corn oil diet, supporting a diet-induced modulation of host inflammatory reactions. Analyses of alveolar bone resorption in the rats related to gene expression profiles demonstrated significant positive correlations with IL-1β, IL-6 and COX-2 and negative correlations with CAT and SOD. Conclusion: These findings suggest that diets enriched for ω-3 FA modulate the local gingival inflammatory milieu of the host following oral P. gingivalis infection, which impacts on alveolar bone resorption in rats.

AB - Introduction: Porphyromonas gingivalis is strongly implicated in the etiology of adult periodontitis by inducing inflammatory cytokines, resulting in gingival and periodontal tissue inflammation and alveolar bone resorption. This study tested the hypothesis that supplementing the diet with omega-3 fatty acid (ω-3 FA; i.e. fish oil) would exert anti-inflammatory effects in the gingival tissues of P. gingivalis-infected rats. Methods: Rats were fed either fish oil or corn oil diets ad libitum for 22 weeks and infected with P. gingivalis strain 381 or strain A7A1-28. After sacrifice, rat gingival tissues were excised and the RNA was isolated and analyzed for proinflammatory mediators [interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), IL-6], T helper type 1 and type 2 cytokines [interferon-γ (IFN-γ), IL-4, IL-10), antioxidant enzymes [catalase (CAT), superoxide dismutase (SOD)], and genes critical for eicosanoid mediator production [cyclo-oxygenase-2 (COX-2), 5-lipoxygenase (5-LO)] by reverse transcription-polymerase chain reaction using rat-specific primers. Results: Rats on the ω-3 FA diet exhibited decreased proinflammatory cytokine gene expression (IL-1β, TNF-α) and enhanced IFN-γ, CAT and SOD messenger RNA expression compared to rats fed a corn oil diet, supporting a diet-induced modulation of host inflammatory reactions. Analyses of alveolar bone resorption in the rats related to gene expression profiles demonstrated significant positive correlations with IL-1β, IL-6 and COX-2 and negative correlations with CAT and SOD. Conclusion: These findings suggest that diets enriched for ω-3 FA modulate the local gingival inflammatory milieu of the host following oral P. gingivalis infection, which impacts on alveolar bone resorption in rats.

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KW - Omega-3 fatty acid

KW - Periodontal disease

KW - Porphyromonas gingivalis

KW - Rat model

KW - Reverse transcription-polymerase chain reaction

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