Oestrogen/progesterone receptors and a 65 kD tumour-associated phosphoprotein (p65) expression in breast cancer patients

H. Niewiadomska, M. Mirowski, J. Bartkowiak, M. Stempien, J. Z. Blonski, Z. Walaszek, M. Hanausek, R. Wierzbicki

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Paraffin-embedded tissue slides fron 89 infiltrating ductal breast carcinoma specimens were assessed immunohistochemically using monoclonal antibodies against human p65 antigen and polyclonal antibodies against p65-like protein present in foetal bovine serum. The p65 expression was correlated with oestrogen receptor (OR) and progesterone receptor (PR) levels estimated immunoenzymatically in the extracts from breast cancer tissue taken after surgery. Statistically significant correlations were found between the relaxed OR, PR state and the cytoplasmic/nuclear positive immunohistochemical reaction with mono-and polyclonal anti-p65 antibodies. It is suggested that the high OR and PR levels are accompanied by the presence of p65 protein in breast cancer tissue which may provide additional evidence identifying the p65 gene as a member of the steroid/ thyroid receptor superfamily.

Original languageEnglish (US)
Pages (from-to)91-99
Number of pages9
JournalBiomedical Letters
Volume56
Issue number222
StatePublished - 1997
Externally publishedYes

Keywords

  • Anti-p65 antibody
  • Breast cancer
  • Oestrogen and progesterone receptor
  • Oncofoetal protein

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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